动物营养学报2018，Vol.30Issue(5)：1981-1987,7.DOI:10.3969/j.issn.1006-267x.2018.05.043
黄曲霉毒素分解酶基因在枯草芽孢杆菌中的表达与应用
Expression and Application of Aflatoxin Detoxification Enzyme Gene in Bacillus subtilis
摘要
Abstract
This experiment was conducted to study the expression and application of aflatoxin detoxification en-zyme(ADTZ)gene in Bacillus subtilis(B.subtilis). The ADTZ gene was transformed into wild-type B.subtilis LN genome by constructing an integrated vector of B.subtilis. The expression of ADTZ protein was detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE). And the recombinant B.subtilis were inoculated in moldy corn as fermentation strains for the fermentation experiment, and the content of aflatoxin B1(AFB1)in moldy corn samples was detected to validate the expression effect of ADTZ gene in B.subtilis. The results showed that the ADTZ gene was successively integrated into wild type B.subtilis genome by con-structing integrative expression vector. The recombined B.subtilis could secrete the specific protein and it was detected by SDS-PAGE. The content of AFB1in the fermented moldy corn of recombined B.subtilis had signifi-cant difference with the fermented moldy corn of uninoculated and wild type B.subtilis LN(P<0.05). It showed that the ADTZ gene is successfully integrated into wild type B.subtilis LN and expressed in extracellu-lar. The expressed product has biological activity,and can degrade AFB1effectively.关键词
黄曲霉毒素分解酶/基因重组/枯草芽孢杆菌/发酵Key words
aflatoxin decomposition enzyme/gene recombination/Bacillus subtilis/fermentation分类
农业科技引用本文复制引用
史敦胜,聂利波,宋洋洋,李元晓,李旺..黄曲霉毒素分解酶基因在枯草芽孢杆菌中的表达与应用[J].动物营养学报,2018,30(5):1981-1987,7.基金项目
国家自然科学基金(31101744) （31101744）
河南省重大科技专项(131100110300) （131100110300）
