福建农业学报2018，Vol.33Issue(3)：225-229,5.DOI:10.19303/j.issn.1008-0384.2018.03.002
短喙型鹅细小病毒感染雏鸭的排毒规律研究
Excretion of SBDS-GPV in Infected Ducklings
摘要
Abstract
Two-day-old mule ducklings were orally inoculated with short beak and dwarfism syndrome goose parvovirus (SBDS-GPV,strain M15) for this study.Swab samples from oropharynx and cloaca of the infected birds were collected after 3,5,7,10,12,14,and 21 days post infection (dPI) for the virus detection by quantitative real time PCR (qPCR),virus isolation (VI),and immunofluorescence (IFA).As determined by qPCR,the excretion of SBDS-GPV through oropharynx occurred in 3-10 dPI,and cloaca,in 3-14 dPI.On the other hand,the VI and IFA tests indicated that the cloacal excretion happened in 5-10 dPI.The results,for the first time,demonstrated that SBDS-GPV could pass through both oropharynx and cloaca of a diseased duckling,and that the greater extent of the excretion took place via cloaca.关键词
半番鸭/短喙矮小综合征(SBDS-GPV)/排毒规律/荧光定量PCR/病毒分离Key words
mule duckling/short beak and dwarfism syndrome goose parvovirus/excretion/qPCR/virus isolation分类
农业科技引用本文复制引用
俞博,林甦,陈仕龙,程晓霞,陈秀琴,陈少莺..短喙型鹅细小病毒感染雏鸭的排毒规律研究[J].福建农业学报,2018,33(3):225-229,5.基金项目
国家重点研发计划(2016YFD0500805-4) （2016YFD0500805-4）
福建省科技计划项目——省属公益类科研院所基本科研专项(2017R1023-1) （2017R1023-1）
