福建农业学报2018，Vol.33Issue(3)：230-235,6.DOI:10.19303/j.issn.1008-0384.2018.03.003
基于线粒体16S rRNA、COX1和Cytb基因探讨11种小丑鱼的系统发育关系
Molecular Phylogeny Determined by Mitochondrial Genes 16S rRNA, COX1 and Cytb of Eleven Anemonefish Species
摘要
Abstract
Sequences of mitochondrial 16S rRNA,COX1 and Cytb genes were determined to establish the phylogenetic relationship among 11 species of anemonefish using the neighbor joining (NJ),maximum-likelihood (ML) and Bayesian methods with the divergence and genetic distances determined as well.The 3 genes had a combined length of 4388 bp that consisted of 1028 polymorphic and 616 parsimonious information sites.The ratio of transition to transversion was 2.12,and the average A+T content was 54.7％,which was higher than 45.3％ for C+G in the genes.It was found that (1) the subfamily Amphiprioninae was monophyly,but the genus Am phiprion was not monophyletic,implying Premnas should be reclassified;(2) the phylogenetic trees grouped the percula complex into a clade that formed the ancestral taxon of anemonefish,but the phylogenetic relationship was uncertain in regard to Premnas biaculeatus;and,while the Bayesian tree placed the percula complex and P.biaculeatus in a same clade,NJ and ML tree classified P.biaculeatus in a clade with subgenera Amphiprion,Paramphiprion and Phalerebus;and,(3) the genetic distance between species suggested a possibility of P.biaculeatus and Premnas epigrammata being identical.关键词
16S rRNA基因/COX1基因/Cytb基因/小丑鱼/遗传差异/系统进化Key words
16S rRNA gene/COX1 gene/Cytb gene/anemonefish/genetic differences/phylogenetic evolution分类
农业科技引用本文复制引用
何丽斌,陈芳,朱志煌,马敏宁,张河长,吴孙龙,周宸..基于线粒体16S rRNA、COX1和Cytb基因探讨11种小丑鱼的系统发育关系[J].福建农业学报,2018,33(3):230-235,6.基金项目
福建省海洋与渔业结构调整专项[闽财指(2016) 841号] （2016）
福建省科技计划项目——省属公益类科研院所基本科研专项(2013R001-7) （2013R001-7）
福州市“十三五”海洋经济创新发展示范项目(FZHJ11) （FZHJ11）
厦门市科学技术局科技计划专项(3502Z20142008) （3502Z20142008）
