福建农业学报2018，Vol.33Issue(3)：258-262,5.DOI:10.19303/j.issn.1008-0384.2018.03.008
花叶金线莲茎段诱导植株再生培养技术研究
Induction Plant Regeneration with Stem Segments of Anoectochilus roxburghii (wall.) lindl.Huaye
摘要
Abstract
Stem segments of Anoectochilus roxburghii (wall.) lindl.Huaye were used as the explants to induce bud clumps.Varied medium (i.e.,MS,B5 or improved MS) and phytohormone (i.e.,TDZ,6-BA,KT or IBA)were applied for the tissue regeneration experiment of an orthogonal design.It was found the major factors affecting the bud multiplication to be basal medium,KT,6-BA and TDZ.The optimal multiplication was achieved by using the improved MS+6-BA with additions of 2.0 mg · L-1 +KT 0.5 mg · L-1 +NAA 0.1 mg · L-1 +sugar 30.0 g · L-1 +agar powder 3.0 g · L-1,and carrageenan 3.6 g · L-1.An averaged propagation coefficient of 4.58 in 60 days was obtained.For rooting,the improved MS+6-BA with added 0.3 mg · L-1 +AC 0.5 g · L-1 +sugar 20.0 g · L-1 +agar powder 3.6 g · L-1 and carrageenan 3.6 g · L-1,rendered a 100.0％ rate in 60 days.关键词
花叶金线莲/丛生芽/正交设计/组织培养Key words
Anoectochilus roxburghii (wall.) lindl.Huaye/bud clumps/orthogonal design/tissue culture分类
农业科技引用本文复制引用
叶秀仙,陈艺荃,钟淮钦,林榕燕,黄敏玲..花叶金线莲茎段诱导植株再生培养技术研究[J].福建农业学报,2018,33(3):258-262,5.基金项目
福建省农业科学院创新团队建设项目(STIT2017-2-9) （STIT2017-2-9）
福建省农业科学院生产性工程化实验室项目(2014GCH-11) （2014GCH-11）
