分析测试学报2018，Vol.37Issue(5)：532-538,7.DOI:10.3969/j.issn.1004-4957.2018.05.003
全二维气相色谱/飞行时间质谱对赤松松针挥发油化学成分的分析
Analysis on Chemical Composition of Volatile Oils in Red Pine Needles by Comprehensive Two-dimensional Gas Chromatography-Time-of-flight Mass Spectrometry
摘要
Abstract
The volatile oils in fresh red pine needles from Xiao Hinggan Mountains,were extracted by steam distillation,and then analyzed by comprehensive two-dimensional gas chromatographytime-of-flight mass spectrometry (GC × GC-TOF MS).The chromatographic separation conditions were optimized,and the best conditions were confirmed to be a modulation period of 6 s,a second dimensional column length of 3 m,and a temperature of 20 ℃ for the second dimensional column higher than that for the first dimensional column all the time.Under the optimal conditions,217 kinds of compounds were identified based the NIST library,and the quantitative calculation was carrled out by area normalization method.Total of the compounds identified was four times more than that identified by one-dimensional gas chromatography (1DGC).124 terpenoids,including 48 monoterpenoids,64 sesquipenoids and 12 diterpenoids were identified from the volatile oils of fresh red pine needles,accounting for about 69％ of all.And 33 ester compounds accounting for about 12％ of all,6 organic acids,15 alcoholic compounds,15 aldehyde compounds,11 ketone compounds and 13 other compounds were also identified.Compaired with the one-dimensional gas chromatography,two-dimensional gas chromatography-time-of-flight mass spectrometry possesses higher resolution and sensitivity,and it shows a unique superiority in the analysis of complex natural products.关键词
松针/挥发油/全二维气相色谱/飞行时间质谱(GC×GC/TOF MS)/化学成分分析Key words
pine needles/volatile oils/comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GC × GC/TOF MS)/analysis of chemical composition分类
化学化工引用本文复制引用
沈正超,孙堂强,胡雅静,杜振霞,杨丽华..全二维气相色谱/飞行时间质谱对赤松松针挥发油化学成分的分析[J].分析测试学报,2018,37(5):532-538,7.基金项目
中央高校基本科研业务费专项资金(ZZ1706) （ZZ1706）
