分析测试学报2018，Vol.37Issue(5)：582-587,6.DOI:10.3969/j.issn.1004-4957.2018.05.010
高效液相色谱法测定化妆品中4种禁用醛酮化合物
Determination of Four Kinds of Prohibited Aldehyde and Ketone Compounds in Cosmetics by High Performance Liquid Chromatography
摘要
Abstract
A high performance liquid chromatographic (HPLC) method was established for the simultaneous determination of four kinds of prohibited aldehyde and ketone compounds (crotonaldehyde,acetophenone,2,4-dihydroxy-3-methylbenzaldehyde and 2-pentylidene cyclohexanone) in cosmetics.The samples were extracted with 50％ acetonitrile solution,then separated on an AQ-C18 (4.6 mm × 150 mm,5 μm) column and detected by HPLC with a diode array detector.The retention time and UV absorption spectra were adopted for qualitation and the external standard method was used for quantitation.The positive cosmetic samples were confirmed by HPLC-MS/MS method.Under the optimal conditions,the results showed that four analytes showed good linearities in the range of 0.05-2.0 mg/L with correlation coefficients not less than 0.999 9.The limits of detection and quantitation were in the ranges of 0.5-1.0 mg/kg and 1.5-3.0 mg/kg,respectively.The mean recoveries for the samples at three spiked levels ranged from 89.0％ to 106.7％ with the relative standard deviations(RSDs,n =6) of 2.0％-6.9％.The method is accurate and reliable,and is suitable for the determination of four kinds of prohibited aldehyde and ketone compounds in cosmetics.关键词
高效液相色谱/化妆品/醛酮化合物Key words
high performance liquid chromatography(HPLC)/cosmetics/aldehydes and ketones分类
化学化工引用本文复制引用
王强,寻知庆,黄金凤,郭新东,吴楚森,叶嘉荣..高效液相色谱法测定化妆品中4种禁用醛酮化合物[J].分析测试学报,2018,37(5):582-587,6.基金项目
国家质检总局科技计划项目(2016QK038) （2016QK038）
