影像科学与光化学2018，Vol.36Issue(3)：236-244,9.DOI:10.7517/j.issn.1674-0475.2018.03.004
基于菁染料高选择性检测半胱氨酸的近红外荧光探针
Near-infrared Cyanine-based Fluorescent Probe for Cysteine with High Selectivity
摘要
Abstract
We developed a turn-on cyanine-based near-infrared (NIR) probe which can selectively detect cysteine.In this probe Cy-CF3,4-(trifluoromethyl)thiophenol moiety is employed as both the recognition unit and fluorescence quenching group.With the titration of cysteine,a large blue shift (160 nm) is observed in the absorption spectra of Cy-CF3,accompanying with significant color changes.The probe Cy-CF3 shows obviously fluorescent enhancement at 780 nm in emission spectra.Over all,the probe Cy-CF3 exhibits high selectivity for cysteine over glutathione and other potential interferences.The reaction mechanism of Cy-CF3 with cysteine is verified by mass spectra,1 HNMR spectra and absorption spectra,indicating that 4-(trifluoromethyl)thiophenol in the probe Cy-CF3 could be replaced by cysteine,and then the PET process from 4-(trifluoromethyl) thiophenol to cyanine is blocked.The cell experiments also demonstrate that this probe could be successfully applied for the detection of cysteine in living cells.关键词
近红外/半胱氨酸/荧光探针/菁染料Key words
near-infrared/cysteine/fluorescent probe/cyanine引用本文复制引用
马亚光,张玉涛,马一宇,郭志前,朱为宏..基于菁染料高选择性检测半胱氨酸的近红外荧光探针[J].影像科学与光化学,2018,36(3):236-244,9.基金项目
国家自然科学基金项目(21622602)资助 （21622602）
