赣南医学院学报2018，Vol.38Issue(3)：205-209,5.DOI:10.3969/j.issn.1001-5779.2018.03.001
茶油抑制实验性结肠炎的作用研究
The Effect of Camellia Oil on the Experimental Colitis
摘要
Abstract
Objective:This project studies whether Camellia oil has a preventive effect on experimental colitis and its mechanisms.Methods:First,C57BL/6 mice were randomly divided into PBS treatment group (Control) and Camellia oil treatment group (Camellia Oil),and then the mice were orally gavaged with Camellia oil for 1 week.After that,mice were treated with 3％ dextran sodium sulfate (DSS).The clinical symptoms of colitis (including weight loss and colon shortening) were observed after treatment.The colonic injury and infiltration of inflammatory cells were observed by H&E staining,and inflammatory cytokines (such as IL-6,MCP-1,KC,and IL-10) were detected by qRT-PCR.Results:Compared with the control group,the mice in the Camellia oil treated group had less weight loss,less c olonic shortening,more intact intestinal mueosal structure,and less infiltration of inflammatory cells after colitis.qRT-PCR results showed that pro-inflammatory cytokines MCP-1 (P ＜ 0.05),KC (P ＜ 0.000 1),and IL-6 (P ＜ 0.05) mRNA levels in colon tissue of mice significantly decreased when compared with untreated group.The anti-inflammatory cytokine IL-10 mRNA levels tended to increase after Camellia oil treatment but there was no statistical significance.Conclusion:Camellia oil has preventive effects on experimental colitis and may inhibit inflammation by downregulating the cytokine expression levels.关键词
茶油/结肠炎/细胞因子Key words
Camellia oleifera/Colitis/Cytokines分类
医药卫生引用本文复制引用
王冰玉,刘丽华,陈亚运,朱鹃花,沈镇平,刘志平..茶油抑制实验性结肠炎的作用研究[J].赣南医学院学报,2018,38(3):205-209,5.基金项目
This work was supported by funds from National Natural Science Foundation of China (31560260),Talents' Startup Fund of Gannan Medical University (QD201404),Camellia Special Project of Gannan Medical University (YC201501),and Collaborative Innovation Center for Gannan Oil-tea Camellia Industrial Development (YK201603) (The above to LIU Zhi-ping),the Undergraduate Science and Technology Innovation Project of Gannan Medical University (To WANG Bing-yu),and the Science and Technology Project from Health and Family Planning Commission of Jiangxi Province (20175550) (To LIU Li-hua) （31560260）
