光学精密工程2018，Vol.26Issue(3)：541-547,7.DOI:10.3788/OPE.20182603.0541
便携式MicroRNA快速检测系统
Portable rapid microRNA detection system
摘要
Abstract
MicroRNA (miRNA) is closely related to the occurrence and development of certain diseases and can be used as a diagnostic marker.Conventional methods for the detection of miRNA are time-consuming and complex.Herein,a portable rapid miRNA detection system was designed.This system records the fluorescence intensity of the marker that is amplified via rolling circle amplification using a photoelectric detection technique,and analyzes the fluorescence data to obtain the changes in miRNA.Through our experiments,we observed that for reagent concentrations in the range 0.1-1 μmol,the linearity of the fluorescence intensity detected by the miRNA fluorescence detection equipment was less than ±5％,and the repeatability was ≥95％.Our roll amplification experiment involving the coronary heart disease diagnostic marker,miR-499,confirmed that the portable rapid miRNA detection system can detect miRNA quickly and effectively.关键词
光电检测/荧光检测/弱光探测/miRNA/滚环扩增Key words
photoelectric detection/fluorescence detection/light detection/miRNA/rolling circle amplification分类
资源环境引用本文复制引用
王燕飞,关一夫,徐赤东,江海河,余东升,陈海燕,张战盈,方蔚恺,杨喆,陆泽橼,李艳蕾,纪玉峰..便携式MicroRNA快速检测系统[J].光学精密工程,2018,26(3):541-547,7.基金项目
国家自然科学基金资助项目(No.81371896) （No.81371896）
