南方农业学报2018，Vol.49Issue(4)：773-779,7.DOI:10.3969/j.issn.2095-1191.2018.04.24
美洲鲥形态特征及其两种同工酶的组织特异性分析
Morphological characteristics of Alosa sapidissima and tissue specificity of two kinds of isozymes in it
摘要
Abstract
[Objective]This paper was to enrich the studies about Alosa sapidissima gerplasm resources from the per-spectives of morphological characteristics and biochemical genetics characteristics,and lay the foundation for screening the biochemical genetic markers that could identify A.sapidissima gerplasm resources.[Method]Countable traits and mea-surable traits of A.sapidissima were determined by morphometry.By using vertical polyacrylamide gel electrophoresis, lactate dehydrogenase(LDH)and malate dehydrogenase(MDH)from six tissues(heart,eye,muscle,liver,kidney and gill)of A.sapidissima were detected and analyzed.[Result]A.sapidissima had a flat fusiform body.Its back and abdomen were shallow arc.And the back were grey and dark,with slight blue-green metallic luster.Both sides of the body and the abdomen were silver white.The fin formulas of A.sapidissima included dorsal fin D.iii-13-15,pectoral fin P.i-14-17,anal fin A.i-ii-16-20,ventral fin V.i-7-9 and caudal fin C.21-25.The morphology of the gill racker was in 7-shape and the num-ber of outer gill racker on the left first gill arch was 24-37+47-64.The isoenzyme band numbers and activity of LDH and MDH from heart,eye,muscle,liver,kidney and gill of A.sapidissima presented tissue specificity and individual varia-tions.[Conclusion]The tissue specificity of A.sapidissima isozymes is regulated and controlled by genetic genes,mean-while it is related to the biochemical metabolism activities of the tissues.It is suggested to choose LDH from gill as bio-chemical genetic markers in A.sapidissima gerplasm identification.关键词
美洲鲥/形态特征/乳酸脱氢酶(LDH)/苹果酸脱氢酶(MDH)/组织特异性Key words
Alosa sapidissima/morphological characteristics/lactate dehydrogenase(LDH)/malate dehydrogenase (MDH)/tissue specificity分类
农业科技引用本文复制引用
张涛,周剑光,陈建武,何力..美洲鲥形态特征及其两种同工酶的组织特异性分析[J].南方农业学报,2018,49(4):773-779,7.基金项目
国家农产品质量安全风险评估重大专项项目(GJFP201700902) （GJFP201700902）
