南方农业学报2018，Vol.49Issue(4)：780-786,7.DOI:10.3969/j.issn.2095-1191.2018.04.25
广西鸡源H9N2亚型禽流感病毒分离鉴定及遗传进化分析
Isolation and identification of H9N2 subtype avian influenza virus from chicken in Guangxi and its genetic evolution
摘要
Abstract
[Objective]This study determined molecular genetic variation regularity of H9N2 subtype avian influenza virus(AIV)and its effects on public health safety of human so as to provide reference for the prevention and control of H9 subtype avian influenza(AI).[Method]Suspected tissue samples were collected from one chicken farm in Guangxi, viral isolation was performed by inoculating SPF chicken embryo.The virus was preliminarily identified as H9 subtype AIV isolated strain.Then eight gene fragments isolated from H9 subtype AIV including HA,NA,PB2,PB,PA,NP,M and NS were sequenced,and their genetic evolution was analyzed.The biological characteristics of the isolated strain were also determined at the same time.[Result]A H9N2 subtype AIV strain,named A/chicken/Guangxi/BT1/2016(H9N2),was successfully separated from the sick chicken population in Guangxi.The cleavage site of HA gene in A/chicken/Guangxi/BT1/2016(H9N2)was PSRSSR↓GLF,which had typical features of low pathogenic AIV(LPAIV)molecule.In genetic evolution relation,it belonged to 4.2.5 branch,and had some differences with the domestic H9N2 subtype AIV vaccine strains which belonged to 4.2.3 branch.The other seven genes were also derived from different AIV subtypes.The isolated virus strain A/chicken/Guangxi/BT1/2016(H9N2)could bind to α-2,3 sialic acid and α-2,6 sialic acid receptors,with the potential risk to infect human. Virus distribution was mainly concentrated in trachea,lung and spleen after it infected chicken,and the virus also could be released through the respiratory tract and digestive tract at same time.The virus was released from day 1 and peaked on the day 5 after the chicken was infected.[Conclusion]A/chicken/Guangxi/BT1/2016 (H9N2),isolated from Guangxi sick chicken population,belonged to LPAIV.It is a H9N2 subtype new virus created by different subtypes AIV.It can lead to the failure of chicken vaccine immunization and has the potential risk of infecting hu-man beings.关键词
禽流感病毒(AIV)/H9N2亚型/分离鉴定/遗传进化/裂解位点/受体结合特性/广西Key words
avian influenza virus(AIV)/H9N2 subtype/isolation and identification/genetic evolution/cleavage site/receptor-binding specificity/Guangxi分类
农业科技引用本文复制引用
张艳雯,何永权,吴云月,于冬玲,曾文榜,宁志鹏..广西鸡源H9N2亚型禽流感病毒分离鉴定及遗传进化分析[J].南方农业学报,2018,49(4):780-786,7.基金项目
病毒学国家重点实验室开放研究基金项目(2016KF008) （2016KF008）
广西高校中青年教师基础能力提升项目(2017KY1443) （2017KY1443）
南宁市科学研究与技术开发计划项目(20172151-1) （20172151-1）
南宁市邕宁区科学研究与技术开发计划项目(20170105C) （20170105C）
