广西医科大学学报2018，Vol.35Issue(5)：608-612,5.DOI:10.16190/j.cnki.45-1211/r.2018.05.006
神经调节蛋白-1对急性心肌梗死大鼠心功能和循环RAS活性的影响
Effects of neuregulin-1 on heart function and circulating RAS activity in acute myocardial infarction rats
摘要
Abstract
Objective:To investigate the influence of neuregulin-1(NRG-1) on ventricular remolding,heart function and the main components of circulating renin-angiotensin-system(RAS) in a rat model of acute myocardial infarction(AMI).Methods:SD rats were randomly divided into the sham group,the model group and the NRG-1 group.Rat models of AMI were established by ligation of the left anterior descending coronary artery in all groups except for the sham group.After 2 hours of modeling,the rats in the NRG-1 group were injected with NRG-1(10 μg/kg) via caudal vein for 7 days,and rats in the sham group and the model group received equal volume of saline.The heart function of the rats was measured by MPA cardiac function analysis system.Myocardial morphology was observed by HE staining.The myocardial fibrosis and collagen volume fraction(CVF) were observed by Masson staining.Serum levels of angiotensin Ⅰ (Ang Ⅰ) and angiotensin Ⅱ (Ang Ⅱ) were detected by enzyme-linked immunosorbent assay(ELISA).Results:Compared with the sham group,the left ventricular systolic pressure(LVSP),and left ventricular pressure decrease maximum rate(-dp/dtmax) were markedly decreased in model group,while the left ventricular end diastolic pressure(LVEDP) was increased significantly(P≥0.01).The LVSP,and-dp/dtmax were elevated,while the LVEDP was declined notably by administrationof NRG-1(P＜0.01).We noticed that model group rats exhibited more severe myocardial fibrosis and inflammatory cell infiltration by HE staining,but reversed by treatment of NRG-1.Moreover,the CVF and the serum levels of Ang Ⅰ and Ang Ⅱ in NRG-1 group were significantly lower than those in the model group (P ＜0.05).Conclusion:NRG-1 inhibited ventricular remodeling and improved heart function through suppressing the activity of RAS.关键词
神经调节蛋白1/急性心肌梗死/血管紧张素Ⅰ/血管紧张素ⅡKey words
neuregulin-1/acute myocardial infarction/angiotensin Ⅰ/angiotensinⅡ分类
医药卫生引用本文复制引用
吴成强,韦静,胡益森,桂春..神经调节蛋白-1对急性心肌梗死大鼠心功能和循环RAS活性的影响[J].广西医科大学学报,2018,35(5):608-612,5.基金项目
国家自然科学基金资助项目(No.81460063) （No.81460063）
广西自然科学基金资助项目(No.2013GXNSFAA019121) （No.2013GXNSFAA019121）
