广西医科大学学报2018，Vol.35Issue(5)：613-617,5.DOI:10.16190/j.cnki.45-1211/r.2018.05.007
表没食子儿茶素没食子酸酯对黄曲霉毒素B1致大鼠急性肝损伤的保护作用
Protective effect of epigallocatechin-3-gallate on acute liver injury induced by aflatoxin B1 in rats
摘要
Abstract
Objective:To study the protective effect of epigallocatechin-3-gallate(EGCG) on acute liver injury induced by aflatoxin B1 (AFB1) in rats.Methods:Fifty SD rats were randomly divided into normal control group,model group,low-and high-dose EGCG intervention groups,and EGCG control group.The rats in the normal control and model group were administrated double distilled water via gavage for 5 days.Rats in the two intervention groups and EGCG control group were administrated 25 mg/kg,100 mg/kg and 100 mg/kg EGCG respectively via gavage for 5 days.On the 6th day,the rats in the control group and EGCG control group were injected intraperitoneally with 0.5 mL DMSO solution,and those in the model group and the two intervention groups were injected intraperitoneally with 2 mg/kg AFB1.The IL-6 level in serum and the 8-OHdG level in liver tissues were measured by enzyme-linked-immunosorbent assay(ELISA).The pathological changes of liver tissues were observed by HE staining.The mRNA expressions of IL-6,Bcl-2 and BAX were determined by qPCR.Results:Compared with the normal control group,pathological changes of hepatic tissues in the model group showed obvious punctate necrosis,hepatocyte ballooning,and inflammatory cell infiltration,whereas a significant improvement in the pathological changes of the livers could be found in the two intervention groups.The protein and mRNA levels of IL-6 as well as the 8-OHdG level in the model group were significantly increased,while the Bcl-2/BAX ratio was decreased(P＜0.05 or P ＜ 0.01).However,the IL-6 mRNA and protein levels and 8-OHdG content were reduced by EGCG administration(P ＜0.01),while the Bcl-2/BAX ratio was elevated (P ＜0.05).No significant differences were noted between the EGCG control group and the normal control group with regard to above indexes(P＞0.05).Conclusion:EGCG could protect from acute liver injury induced by AFB1 in rats,and the mechanism might be related to attenuating inflammatory response,reducing hepatocyte DNA injury and inhibiting cell apoptosis.关键词
表没食子儿茶素没食子酸酯/黄曲霉毒素B1/急性肝损伤/大鼠Key words
epigallocatechin-3-gallate/aflatoxin B1/acute liver injury/rat分类
医药卫生引用本文复制引用
邓志杰,赵静芳,肖德强,黄丰,张勇胜,刘丹丹,高伟,黄东萍,鲁力..表没食子儿茶素没食子酸酯对黄曲霉毒素B1致大鼠急性肝损伤的保护作用[J].广西医科大学学报,2018,35(5):613-617,5.基金项目
国家自然科学基金资助项目(No.31360383) （No.31360383）
