广西医科大学学报2018，Vol.35Issue(5)：618-622,5.DOI:10.16190/j.cnki.45-1211/r.2018.05.008
维生素C对Treg/Th17失衡的调节作用
The effect of vitamin C on Treg/Th17 imbalance
摘要
Abstract
Objective:To study the effect of vitamin C(Vit C) on the imbalance of Treg/Th17 in EL4 cells.Methods:The proliferation rate of EL4 cells treated by different concentrations of Vit C or prednisone was detected by CCK-8.Transforming growth factor(TGF)-β1,interleukin(IL)-6 and IL-23 were used to induce CD4+T cells differentiation into Th17.The mRNA expressions of retinoic acid receptor-related orphan nuclear receptor gamma t(RORγt) and fork head box protein-3(Foxp3) were determined by qPCR.The levels of IL-17A and IL-10 in culture supernatant were detected by enzyme-linked immunosorbent assay(ELISA).Results:Both 0.2 mmol/L Vit C and 1 μmol/L prednisone had no obvious effect on the proliferation of EL4 cells,and could lead to increased expressions of RORγt and Foxp3,but a decreased secretion of IL-10(P＜0.05).Conclusion:Vit C has a similar regulation effect on Treg/Th17 imbalance to that of prednisone(1 μmol/L),and there is a correlation between the intensity of the action and time.关键词
维生素C/EL4细胞/Foxp3/RORγt/IL-10/IL-17AKey words
vitamin C/EL4 cell/Foxp3/RORγt/IL-10/IL-17A分类
医药卫生引用本文复制引用
李东鞠,符晓倩,李宁,吴艳凤,李慕军..维生素C对Treg/Th17失衡的调节作用[J].广西医科大学学报,2018,35(5):618-622,5.基金项目
国家自然科学基金资助项目(No.81360101) （No.81360101）
广西医疗卫生适宜技术研究与开发资助项目(No.S2015 26) （No.S2015 26）
