广西医科大学学报2018，Vol.35Issue(5)：643-647,5.DOI:10.16190/j.cnki.45-1211/r.2018.05.014
腱周组织包绕对重建后交叉韧带移植物组织学及血管内皮因子的影响
The autograft histological changes and VEGF level after PCL reconstruction using flexor hallucis longus tendons wrapping with peritendon tissues
摘要
Abstract
Objective:To investigate the histological changes of autograft and vascular endothelial growth factor(VEGF) level after posterior cruciate ligament (PCL) reconstruction using flexor hallucis longus (FHL) tendons wrapping with peritendon tissues.Methods:PCL was reconstructed with FHL wrapping the peritendon tissues of Achilles tendons in 24 Beagle dogs(experimental group) and with simple tendon autograft(control group).After 4,8 and 12 weeks,the histological changes of autograft were observed,and VEGF protein expression was detected by immunohistochemistry.Results:In the experimental group,the grafted tendons were surrounded by granulation tissues with abundant blood vessels and a large number of fibroblasts around at 4 weeks post-operation.After 8 weeks,the synovium formed and the ligaments were similar to the normal PCL.At week 12,the synovium around the grafts became thinner.Under the microscope,the fibrocyte nuclei in the grafted ligaments gradually became rod-like and spindle-like shapes and well-organized,which was the same as the normal PCL.In comparison,these changes in the control group were much slower.There was a significant difference in the average optical density of VEGF between the two groups at 4 weeks post-operation(P＜0.05),and the changes were more significant at week 8 and 12(P ＜0.01).Conclusion:Application of the additional peritendon tissues of Achilles tendons wrapping autograft in PCL reconstruction could enhance VEGF level,facilitate vascularization,and promote fibrocyte migration and proliferation in grafts.关键词
血管内皮因子/后交叉韧带重建移植物/免疫组化/平均光密度Key words
vascular endothelial growth factor/posterior cruciate ligament reconstruction/immunohistochemistry/average optical density分类
医药卫生引用本文复制引用
张冉,沙亮..腱周组织包绕对重建后交叉韧带移植物组织学及血管内皮因子的影响[J].广西医科大学学报,2018,35(5):643-647,5.基金项目
柳州市科技计划资助项目(No.2015J030524) （No.2015J030524）
