广西医科大学学报2018，Vol.35Issue(5)：648-651,4.DOI:10.16190/j.cnki.45-1211/r.2018.05.015
新生小鼠Sca-1+心脏干细胞的分离与鉴定
Isolation and identification of Sca-1 positive cardiac stem cells in newborn mice
摘要
Abstract
Objective:To isolate and purify the stem cell antigen 1(Sca-1) positive cardiac stem cells from mouse,and to observe its specific markers.Methods:Mice cardiac stem cells were generated with tissue block culture method.After 10-14 days,Sca-1 positive cardiac stem cells were isolated by magnetic cell sorting(MACS),and identified by flow cytometry and immunofluorescence,respectively.Cardiac-specific transcription factors GATA-4 and Nkx-2.5 were detected by immunofluorescence.Results:Sca-1 positive cardiac stem cells were successfully separated and purified.Flow cytometry demonstrated that the proportion of Sea-1 positive cardiac stem cells was(62.8±1.3)％.Immunofluorescence showed positive expressions of Sca1,GATA-4 and Nkx-2.5.Conclusion:Sca-1 positive cardiac stem cells in newborn mice could be isolated by MACS and stably cultured.The cardiac-specific transcription factors GATA-4 and Nkx-2.5 could be detected.关键词
新生小鼠/Sca-1+心脏干细胞/干细胞抗原1/磁珠分选/流式细胞术/鉴定Key words
newborn mice/Sca-1 positive cardiac stem cell/stem cell antigen-1/magnetic cell sorting/flow cytometry/identification分类
医药卫生引用本文复制引用
韦静,吴成强,桂春..新生小鼠Sca-1+心脏干细胞的分离与鉴定[J].广西医科大学学报,2018,35(5):648-651,4.基金项目
广西自然科学基金资助项目(No.2013GXNSFAA019121) （No.2013GXNSFAA019121）
