河北医学2018，Vol.24Issue(5)：794-797,4.DOI:10.3969/j.issn.1006-6233.2018.05.024
丝胶通过调节JNK信号通路抑制糖尿病大鼠视网膜神经细胞凋亡
Sericin Inhibits Retinal Neuron Apoptosis in Diabetic Rat via Regulation of JNK Signaling Pathway
摘要
Abstract
Objective:This study was to investigate the effects of sericin on the inhibition of retinal neu-ron apoptosi via regulation of JNK signaling pathway in diabetic rat.Methods:The diabetic rat models were es-tablished by feeding high lipid foods and intraperitoneal injection of small-dose streptozotocin. Rats were ran-domly divided into three groups, sericine-treatment group, diabetes model group and normal control group. The sericin solution dissolved in normal saline solution 2.4 g·kg-1·d-1was used for 35 days in gavage meth-od in the sericin-treated group. The rats were sacrificed by an overdose of anesthesia and retinal sections were prepared. The expressions of phosphorylation c-Jun N-terminal kinase( p-JNK),Cytochrome(Cyt-c)and Cysteine aspartate kinase 9(caspase-9)in retinas in protein levels were detected by Western blot.The activity of Cysteine aspartate kinase 3(caspase-3)in the retina was detected by spectrophotometry. TUNEL staining was employed to detect retinal neuron apoptosis. Results:The relative expression levels of p-JNK,Cyt-c and caspase-9 proteins, the activity of caspase-3,the apoptotic index(AI) in rat retinas in the sericintreated group were significantly lower than those in the diabetic model group (all at P<0.05). Conclusion:Sericin can inhibit retinal neuron apoptosis by regulating JNK signaling pathway in diabetic rats.关键词
丝胶/药物/糖尿病视网膜病变/c-Jun氨基末端激酶/信号通路/凋亡Key words
Sericins/pharmacology/Diabetic retinopathy/c-Jun N-terminal kinase/Signaling pathway/Apoptosis引用本文复制引用
董志军,陈志宏,陶相宜,于宏飞,杨帆..丝胶通过调节JNK信号通路抑制糖尿病大鼠视网膜神经细胞凋亡[J].河北医学,2018,24(5):794-797,4.基金项目
国家自然科学基金项目,(编号:81441133) （编号:81441133）
河北省自然科学基金项目,(编号:H2013406096) （编号:H2013406096）
河北省承德市科技计划项目,(编号:201606A056) （编号:201606A056）
