郑州大学学报（医学版）2018，Vol.53Issue(3)：269-271,3.DOI:10.13705/j.issn.1671-6825.2017.10.047
Mcl-1 siRNA转染对EC9706细胞紫杉醇化疗敏感性的影响
Effect of Mcl-1 siRNA transfection on sensitivity of EC9706 cells to pa-clitaxel
摘要
Abstract
Aim:To observe the sensitivity of esophageal cancer EC9706 cells to paclitaxel(PTX) after Mcl-1 RNA interference. Methods:Western blot was used to detect the expression of Mcl-1 protein in normal esophageal epithelial Het-1 cells and esophageal carcinoma EC9706 cells. EC9706 cells transfected with or without Mcl-1 siRNA were treated with different concentrations of PTX for 48 h,and the changes in cell proliferation were observed by MTT assay. EC9706 cells transfected with or without Mcl-1 siRNA were treated with 0.4 μmol/L PTX for 24 h,and the apoptosis was examined by flow cytometry.Results:Compared with Het-1 cells, Mcl-1 protein was highly expressed in EC9706 cells. Compared with the EC9706 cells treated by PTX alone,those treated by PTX combined with Mcl-1 siRNA transfection were more sen-sitive to PTX with higher proliferation inhibition rate and apoptosis rate(P<0.05). Conclusion:The interference of anti-apoptosis gene Mcl-1 can increase the sensitivity of EC9706 cells to PTX.关键词
Mcl-1蛋白/EC9706细胞/RNA干扰/紫杉醇Key words
Mcl-1 protein/EC9706 cell/RNA interference/paclitaxel分类
医药卫生引用本文复制引用
秦甜甜,刘卫华,张雪燕,李蕾蕾,霍俊峰,石晓丽,王淙..Mcl-1 siRNA转染对EC9706细胞紫杉醇化疗敏感性的影响[J].郑州大学学报（医学版）,2018,53(3):269-271,3.基金项目
国家自然科学基金面上项目(81430085) （81430085）
2015年度河南省基础与前沿技术研究(152300410030) （152300410030）
河南省高等学校重点科研项目(18A350013,14B350011) （18A350013,14B350011）
