郑州大学学报（医学版）2018，Vol.53Issue(3)：316-319,4.DOI:10.13705/j.issn.1671-6825.2017.10.018
NOX4在BEAS-2B细胞上皮间质转化过程中的作用
Role of NOX4 in epithelial-mesenchymal transition process of BEAS-2B cells
摘要
Abstract
Aim:To explore the role of non-phagocytic oxidase 4(NOX4) in the epithelial-mesenchymal transition(EMT) of BEAS-2B cells.Methods:BEAS-2B cells at logarithmic growth phase were allocated into 4 groups,blank control group,TGF-β group,DPI(an inhibitor of NOX4) group,and TGF-β+DPI group. Western blot was used to detect the expression of E-cad-herin,Vimentin and NOX4. The cell migration ability was detected by scratch experiment. The reactive oxygen(ROS) was de-tected by flow cytometry.Results:It was shown that TGF-β could increase the expression of NOX4,Vimentin and ROS,and re-duce the expression of E-cadherin and the width of scratches(P<0.001). DPI could inhibit the expression of NOX4,Vimentin and ROS,and increase the expression of E-cadherin and the width of scratches(P<0.001). The combination of DPI and TGF-β had antagonistic effects(P<0.05).Conclusion:Under the experimental conditions,inhibition of NOX4 expression can inhibit TGF-β-induced BEAS-2B cells'EMT process,thus inhibit the migration of BEAS-2B cells.关键词
BEAS-2B细胞/非吞噬细胞氧化酶4/上皮间质转化/肺上皮细胞Key words
BEAS-2B cell/non-phagocytic oxidase 4/epithelial-mesenchymal transition/pulmonary epithelial cell分类
医药卫生引用本文复制引用
薛腾,赫欣,任亚南,张恺,阙菡雅,姚武,周舫..NOX4在BEAS-2B细胞上皮间质转化过程中的作用[J].郑州大学学报（医学版）,2018,53(3):316-319,4.基金项目
河南省科技攻关项目(172102310267) （172102310267）
河南省高等学校重点科研项目(16A330008) （16A330008）
