河南农业科学2018，Vol.47Issue(3)：34-37,4.DOI:10.15933/j.cnki.1004-3268.2018.03.007
郑麦9023胚性悬浮细胞系的建立和植株再生
Establishment of Embryogenic Suspension Cell and Plant Regeneration of Wheat Zhengmai 9023
摘要
Abstract
In order to establish a rapid,efficient and stable genetic transformation system for wheat,rataria of wheat Zhengmai 9023 were cultured to induce calluses,and the effect of different concentrations of 2,4-D and KT in mediums on callus induction and subculture was studied.The results showed that the optimal callus induction medium was MS+ 3.0 mg/L 2,4-D,and the highest ratio of callus induction was 92.5%;the optimal medium for callus subculture was MS+ 1.0 mg/L 2,4-D+0.1 mg/L KT;the optimal medium for cell suspension was MS +1.0 mg/L 2,4-D + 0.1 mg/L KT;the optimal time for subculture of suspension system was 16 d.The mature embryos in suspension system could develop into normal plants in the basic medium.The embryogenic cell suspension could be used for genetic engineering and screening of somaclonal mutants in wheat.关键词
小麦/郑麦9023/胚性悬浮细胞系/植株再生Key words
Wheat/Zhengmai 9023/Embryogenic suspension cell/Plant regeneration分类
农业科技引用本文复制引用
李冬兵,吕桂珍,牛洪斌,尹钧..郑麦9023胚性悬浮细胞系的建立和植株再生[J].河南农业科学,2018,47(3):34-37,4.基金项目
"十二五"国家科技支撑计划重大项目(2011BAD16B07) （2011BAD16B07）
