河南农业科学2018，Vol.47Issue(3)：138-143,6.DOI:10.15933/j.cnki.1004-3268.2018.03.027
鸭源鸡杆菌flfA基因的克隆及功能区域分析
Cloning of the flfA Gene of Gallibacterium anatis and Analysis of Its Function Domain
摘要
Abstract
The aim of this study was to understand the distribution and function of fimbriae FlfA protein of G.anatis.flfA genes were amplified from 18 G.anatis isolates,followed by molecular cloning,bioinformat-ics analyses of flfA nucleotide and amino acid sequences with bioinformatics tools.The results showed that the nucleotide sequences homologies of 18 G.anatis isolates were 70.2%—99.8% and the deduced ami-no acid homologies were 63.1%—98.8%.FlfA protein has 6—8 B cell epitopes and most of them were located in the conserved sequence region.These results indicate that different strains may show immune cross protection.关键词
鸭源鸡杆菌/flfA基因/克隆/序列分析Key words
Gallibacterium anatis/flfA gene/Cloning/Sequence analysis分类
农业科技引用本文复制引用
王坤芃,彭志锋,刘盼盼,王继洋,王艳,王川庆,杨霞..鸭源鸡杆菌flfA基因的克隆及功能区域分析[J].河南农业科学,2018,47(3):138-143,6.基金项目
河南省自然科学基金资助项目(162300410153) （162300410153）
河南省高校科技创新团队与支持计划资助项目(14IRTSHN015) （14IRTSHN015）
国家自然科学基金项目(U1404328) （U1404328）
