杭州师范大学学报（自然科学版）2018，Vol.17Issue(2)：143-145,152,4.DOI:10.3969/j.issn.1674-232X.2018.02.006
HPLC法测定苦蘵中酸浆苦味素D的含量
Determination of Physalin D in Physalis Angulata L. by High Performance Liquid Chromatography
摘要
Abstract
A method was established for the determination of physalin D in the calyx of Physalis angulata L.A Waters Symmetry C18column(4.6×250 mm,5 μm)and mobile phase of acetonitrile-water(25:75)as well as 227 nm UV absorption at 25 ℃,by high performance liquid chromatography.The calibration curve of physalin D was in good linear relationship over the range of 10~100 μg·mL-1(r=0.999 8).The average recovery was 98.7%,and RSD(n=3)was 1. 8%.Therefore,this method is simple,quick,sensitive,accurate,and reproducible and can could be used to control the quality of Physalis angulata L.关键词
酸浆苦味素D/高效液相色谱法/苦蘵Key words
physalin D/HPLC/Physalis angulata L.分类
医药卫生引用本文复制引用
展晓日,方雷,廖昕玥,俞春娜,沈丽燕,冯尚国,沈晨佳,王慧中..HPLC法测定苦蘵中酸浆苦味素D的含量[J].杭州师范大学学报（自然科学版）,2018,17(2):143-145,152,4.基金项目
国家自然科学基金项目(31470407) （31470407）
浙江省科技计划项目(2014C32090) （2014C32090）
浙江省新苗人才计划(2017R423081) （2017R423081）
杭州市科技项目(20150932H04) （20150932H04）
杭州市科技项目(20150932H03). （20150932H03）
