杭州师范大学学报（自然科学版）2018，Vol.17Issue(2)：159-165,7.DOI:10.3969/j.issn.1674-232X.2018.02.009
外源硼酸抑制柑橘采后绿霉病害Penicillium digitatum的效果研究
Inhibitory Effect of Exogenous Boric Acid on Postharvest Disease Penicillium Digitatum of Citrus
摘要
Abstract
Penicillium digitatum is a major postharvest pathogen and is quite harmful to fruit and vegetable preservation.Therefore,it is necessary to search for an effective control method.In this study,the main pathogen is isolated from naturally infected citrus and identified by rDNA-ITS method.Then the minimum inhibitory concentrations (MICs)of exogenous boric acid(BA)were confirmed.The relationships between exogenous BA as well as the growth and pathogenicity of the pathogen were evaluated via the assessments of physiological,biochemical indexes and inoculation experiments of fruits.The results indicated that the isolated pathogen was identified as P.digitatum.The antifungal effect of BA had positive relationship with its concentration.Under the MICs,BA could significantly inhibit spore germination, reduce germ tube elongation and mycelium extension rate,lower biomass accumulation,result in sugar malabsorption of P.digitatum,and effectively control green mold of citrus caused by P.digitatum.This study provided a new approach to control P.digitatum.关键词
硼酸/柑橘/Penicilliumdigitatum/抑菌作用/贮藏保鲜Key words
boric acid/citrus/Penicillium digitatum/antifungal activity/preservation and freshness分类
农业科技引用本文复制引用
罗锦,王小红,叶必顺,闫芝茜,刘颖,周丽琬,周婷..外源硼酸抑制柑橘采后绿霉病害Penicillium digitatum的效果研究[J].杭州师范大学学报（自然科学版）,2018,17(2):159-165,7.基金项目
国家自然科学青年基金项目(31501810) （31501810）
国家级大学生创新创业训练计划项目(201710346007) （201710346007）
杭州市农业科研攻关项目(20140432B02). （20140432B02）
