杭州师范大学学报（自然科学版）2018，Vol.17Issue(2)：166-169,176,5.DOI:10.3969/j.issn.1674-232X.2018.02.010
绿脓菌素诱导NK92细胞线粒体损伤的研究
On the Mitochondrial Damage Induced by Pyocyanin in NK92 Cells
摘要
Abstract
Pyocyanin(PCN)produced by Pseudomonas aeruginosa induces the apoptosis of NK92 cells.Investigate the molecular mechanism of which PCN induces apoptosis in natural killer cells.NK92 cells are stimulated by PCN,and then the mitochondrial membrane potential and pro-apoptotic protein expression are evaluated by flow cytometry and Western blot. The results show that with the concentration of PCN stimulation increasing,about 30% of the NK92 cells mitochondrial membrane potential decrease,and the pro-apoptotic protein Bak,Puma,Phospho-Bad and Bik are activated by PCN in NK92 cells.It is concluded that PCN can activate pro-apoptotic proteins,and cause the damage of the mitochondria and apoptosis in NK92 cells.关键词
绿脓菌素/自然杀伤细胞/促凋亡蛋白/线粒体损伤Key words
Pyocyanin/NK cell/pro-apoptotic protein/mitochondrial damage分类
生物科学引用本文复制引用
袁喆晨,黄小园,汪林芳,金丽婷,姚悦,徐怡冉,朴正浩..绿脓菌素诱导NK92细胞线粒体损伤的研究[J].杭州师范大学学报（自然科学版）,2018,17(2):166-169,176,5.基金项目
浙江省自然科学基金项目(KZ13058) （KZ13058）
杭州师范大学"星光计划"2017年大学生创新创业项目.*具有同等贡献. （）
