家畜生态学报2018，Vol.39Issue(4)：43-48,6.
不同月龄阶段陕北白绒山羊生精细胞发育规律研究
Histological Analysis of Testis in Shanbei White Cashmere Goats at Different Developmental Stages
摘要
Abstract
In order to explore the spermatogenic cell development of Shaanbei white cashmere goats at different developmental stages,the testis of Shaanbei white cashmere goats (one-,three-,six-month-old and adult goats) were collected and cut into pieces for staining using hematoxylin-eosin.The results showed that (1) The diameter of goat seminiferous tubules increased significantly with age (P＜0.05).The difference in diameter between 3-month-old and adult goats was not significant,representing 150.72 μm and 161.94 μm,respectively(P＞0.05).(2)The cell layer of goats also increased with age,the cell layer of 6-month-old goat (7.70 layers) was significantly higher than that of one-and three-month-old goats,respectively (P＜0.05).The total number of cross-sectional cells in seminiferous tubules both of six-month-old and adult goats was strikingly different compared to those of 1-month-old and 3-month-old cashmere goats (P ＜ 0.05).(3) Moreover,the proportion of spermatogonia of 3-month-old goats (46.40％) was significantly higher than that of one-,six-month-old and adult goats,just about 33.10％,9.10％ and 7.60％,respectively (P＜0.05).(4)The expression of PLZF in testis decreased significantly with age (P＜0.05).Marker genes of SSCs,such as CD49f,PLZF,Thy1,ETV5,VASA,Oct4,Nanog and Nanos,were highly expressed in the testis of 3-month-old goats,and except for the CD49f gene,expression levels of other genes were significantly higher than those of other groups (P＜0.05).The results showed that the cashmere goats reached sexual maturity in 6 month old,and the most abundant spermatogonia were in 3-month-old testis.关键词
陕北白绒山羊/生精细胞/发育规律/精原细胞Key words
Shanbei white cashmere goat/germ cell/development law/spermatogonial cells分类
农业科技引用本文复制引用
韩聪,方乾,李浩,胡姗,朱海鲸,胡建宏..不同月龄阶段陕北白绒山羊生精细胞发育规律研究[J].家畜生态学报,2018,39(4):43-48,6.基金项目
国家自然科学基金项目(31672425) （31672425）
