家畜生态学报2018，Vol.39Issue(4)：49-54,6.
体外受精生产性控犏牛胚胎条件的优化
Optimization of the Production Conditions for Sexed Embryos of Yak Cattle by in vitro Fertilization
摘要
Abstract
In order to improve the productivity of sexed Yak cattle embryos in vitro,this experiment was conducted to optimize the links from sex-sorted sperm concentration,fertilization time,degree of removal cumuluscells,different embryo culture system and embryo culture environment.The results showed that:(1) the cleavage rate and blastocyst rate of fertilization 24 h was significantly higher than that of 6 h and 18 h (P＜0.05);(2) The cleavage rate of sex-sorted sperm concentration of 4～6 × 106/mL was significantly higher than that of 0.4～0.6 × 106/mL (P＜0.05),but blastocyst rate difference was insignificant (P＞0.05),which were all significantly higher than the concentration of 0.04～0.06 × 106/mL (P＜0.05);(3) The blastocyst rate of intact cumulus cells group was significantly higher than partial removal cumulus cells group and complete removal cumulus cells group(P＜0.05);(4) The blastocyst rate of coculture with oviductal epithelial cells (24.63 ％) and cumulus cells (23.42 ％) were of no significant difference,but they were significantly higher than FBS CR1aa medium (P＜0.05);(5)The sex-control blastocyst cleavage rate in low oxygen group (23.42％) was significantly higher than high oxygen group (11.83％) (P＜0.05),but the hatched blastocyst rates were not significantly different.The results indicated that sex sperm concentration of 0.4 ～0.6 × 106/mL,24h fertilization time,intact cumulus cells method,co-culture with oviductal epithelial cells,embryo embryonic environment with low oxygen environment were the best condition of Yak cattle embryo production.关键词
犏牛/体外受精/性控精液/卵母细胞/氧浓度Key words
Yak cattle embryo/in viro fertilization/sex-sorted sperm/oocytes/oxygen density分类
农业科技引用本文复制引用
王森,孙永刚,李瑞哲,马志杰,陈生梅,徐惊涛..体外受精生产性控犏牛胚胎条件的优化[J].家畜生态学报,2018,39(4):49-54,6.基金项目
青海省科技厅科技创新能力促进计划(2015-ZJ-712) （2015-ZJ-712）
科技部国际科技合作项目(2013DFA31420) （2013DFA31420）
