解放军医学杂志2018，Vol.43Issue(4)：289-293,5.DOI:10.11855/j.issn.0577-7402.2018.04.04
右美托咪定对脂多糖刺激后大鼠肾上腺嗜铬细胞瘤细胞的保护作用及机制
Protective role and mechanism of dexmedetomidine on the LPS-stimulated PC12 cells
摘要
Abstract
Objective To explore the effect ofdexmedetomidine on the lipopolysaccharide (LPS) stimulated PC12 cells and its potential mechanism.Methods PC12 cells were treated by LPS with a concentration of 400μg/ml.The cell viability,the concentrations ofinterleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) in the cell culture supernatant were measured after 3-,6-,or 12-h treatment.The expressions of toll-like receptor 4 (TLR4),myeloid differentiation factor 88 (MyD88) and phosphorylated p65 (p-p65) were measured.In the second part,PC12 cells were cultured under four different treatments,that is,normal culture media in first group,400μg/ml LPS in second group,100μmol/L dexmedetomidine in third group,400μg/ml LPS and100μmol/L dexmedetomidine in fourth group.The indexes mentioned above were measured 6 hours after LPS and DEX treatments.Results The cell viability was decreased after LPS treatment,and the concentrations of IL-6 and TNF-α were increased significantly.Compared with control group,the concentrations in 3-,6-,12-h groups showed statistically significant differences (P＜0.05),especially after 6 hours.The TLR4/MyD88/NF-κB pathway was activated after LPS stimuli and reached the peak value.Compared with LPS treatment group,PC 12 cell apoptosis rate,the concentrations of IL-6 and TNF-α and the expressions of TLR4,MyD88 and p-p65 were decreased.The differences between LPS+DEX group and LPS group was statistically significant (P＜0.05).Conclusion Dexmedetomidine has a protective effect on LPS stimulated PC 12 cells via the inhibition of inflammatory response.关键词
脂多糖/右美托咪定/PC12细胞/Toll样受体4Key words
lipopolysaccharide/dexmedetomidine/PC12 cells/Toll-like receptor 4分类
医药卫生引用本文复制引用
周俊杰,肖伟,何璇,彭娜,童华生,苏磊..右美托咪定对脂多糖刺激后大鼠肾上腺嗜铬细胞瘤细胞的保护作用及机制[J].解放军医学杂志,2018,43(4):289-293,5.基金项目
广东省自然科学基金博士启动项目(2016A030310288).This work was supported by the Doctoral Start-up Projects of Natural Science Foundation of Guangdong Province (2016A030310288) （2016A030310288）
