解放军医学杂志2018，Vol.43Issue(4)：310-315,6.DOI:10.11855/j.issn.0577-7402.2018.04.08
17-AAG在氧糖剥夺后复糖复氧诱导的神经元凋亡中的作用
Effects of 17-AAG on neurons apoptosis induced by oxygen-glucose deprivation and recovery
摘要
Abstract
Objective To explore the role and mechanism of 17-allylamino-17-demethoxygeldanamycin (17-AAG) in neurons apoptosis induced by oxygen-glucose deprivation and recovery (OGD/R).Methods Primary rat neurons were cultivated in vitro,and OGD/R model was reproduced.Neurons were exposed to OGD for 0.5h,1h and 2h,and then reperfusion for 24h,the effect of OGD/R on neurons apoptosis was detected by TUNEL assay.On OGD/R,neurons were treated with different concentrations of 17-AAG (0.5,1.0 and 2.0μmol/L),and the effect of 17-AAG on OGD/R treated neurons apoptosis was detected by TUNEL assay.Western blotting was performed to detect the expression of heat shock protein 70 (HSP70).HSP70 interference lentivirus was then constructed.The effect of HSP70 interference on the neurons apoptosis treated with OGD/R and 17-AAG was detected by TUNEL assay.Results OGD/R significantly induced neurons apoptosis,and the rate of neurons apoptosis increased with the increase of OGD time.17-AAG obviously inhibited the neurons apoptosis induced by OGD/R,and the higher the 17-AAG concentration,the more obvious the inhibition.OGD/R significantly suppressed the expression of HSP70 protein in neurons,and 17-AAG obviously reversed the inhibition of HSP70 protein expression induced by OGD/R.However,HSP70 lentivirus interference markedly reversed the protective effect of 17-AAG on OGD/R treated neurons.Conclusion 17-AAG may inhibit the apoptosis of OGD/R treated neurons by up-regulating HSP70.关键词
17-AAG/热休克蛋白70/神经元/细胞凋亡Key words
17-AAG/heat shock protein 70/neuron/apoptosis分类
医药卫生引用本文复制引用
李建雄,李鸣明,卜玉洁,李斌,邹华,张廷华..17-AAG在氧糖剥夺后复糖复氧诱导的神经元凋亡中的作用[J].解放军医学杂志,2018,43(4):310-315,6.基金项目
甘肃省兰州市科技计划项目基金(2017-2-70).This work was supported by the Science and Technology Fund of Lanzhou City,Gansu Province (2017-2-70) （2017-2-70）
