解放军医学杂志2018，Vol.43Issue(4)：322-327,6.DOI:10.11855/j.issn.0577-7402.2018.04.10
缺氧环境下自噬对内皮祖细胞迁移及凋亡的影响
Effects of autophagy on cell migration and apoptosis of endothelial progenitor cells in hypoxic environment
余森 1杨杰 1张继航 1黄岚1
作者信息
- 1. 400037重庆 陆军军医大学新桥医院全军心血管病研究所
- 折叠
摘要
Abstract
Objective To investigate the effects of autophagy on cell migration and apoptosis of endothelial progenitor cells (EPCs) in hypoxic environment.Methods Rats' bone marrow-derived EPCs were isolated by density-gradient centrifugation,identified with double-fluorescent staining method,and then divided into 4 groups:normoxia (hypoxia 0h),hypoxia 1h,hypoxia 3h and hypoxia 6h groups for detecting the effects of hypoxia on EPCs migration and apoptosis.Transwell chambers and flow cytometry were used to detect cell migration and apoptosis respectively,and Western blotting was performed to detect the changes of autophagy-related proteins,including microtubule associated protein 1 light chain 3 (LC3-Ⅱ / Ⅰ) and ubiquitin of p62.To investigate the effects of hypoxic-induced autophagy on EPCs migration and apoptosis,cells were divided into 4 groups:hypoxia 0h,hypoxia 3h,hypoxia 3h+ShAtg7 and ShAtg7 groups,and then to detect again the EPCs migration and apoptosis.Results Compared to hypoxia 0h group,the EPCs migration markedly reduced and apoptosis increased in hypoxic 3h and 6h groups with statistically significant difference (P＜0.05).Western blotting showed the expressions of LC3-Ⅱ / Ⅰ significantly increased and of p62 decreased in EPCs by hypoxic treatment for 3 and 6 hours (P＜0.05),implying the increases of autophagy level.After inhibiting autophagy by silencing Atg7,hypoxia 3h+ShAtg7 group further reduced EPCs migration (P＜0.05) and aggravated apoptosis (P＜0.05) compared with hypoxia 3h group.Conclusion Autophagy may play a cytoprotective role through promoting EPCs migration and decreasing EPCs apoptosis in hypoxic environment.关键词
内皮祖细胞/自噬/缺氧/迁移/凋亡Key words
endothelial progenitor cells/autophagy/hypoxia/cell migration/apoptosis分类
医药卫生引用本文复制引用
余森,杨杰,张继航,黄岚..缺氧环境下自噬对内皮祖细胞迁移及凋亡的影响[J].解放军医学杂志,2018,43(4):322-327,6.
