检验医学与临床2018，Vol.15Issue(10)：1428-1430,3.DOI:10.3969/j.issn.1672-9455.2018.10.017
CarbAcineto NP快速检测耐碳青霉烯酶的鲍曼不动杆菌的方法学评价
CarbAcineto NP method for rapid detection carbapenemase producing in acinetobacter baumannii
牛翠 1黄红革 2马芳 1薛云 1董月稳 1时东彦1
作者信息
- 1. 河北省邢台市第三医院检验科 054000
- 2. 河北医科大学第二附属医院检验科,石家庄050000
- 折叠
摘要
Abstract
Objective To evaluate the method of fast detecting carbapenemase of acinetobacter baumannii. by CarbAcineto NP.Methods A total of 80 strains were used to evaluate the performance of test from Januar-y 2015 to January 2016 in the Third Hospital of Hebei Xing Tai.Identification and susceptibility testing of 80 strains of A.baumannii was determinated by VITEK compact.CarbAcineto NP was applied to detect the Car-bapenemase of the A.baumannii and the OXA-23 gene of the Carbapenemase was detected by the common PCR method.Results There were 49 of 80 strains resistant to imipenem and meropenem.PCR showed that 47 of 49 were carrying OXA-23 gene,43 of 49 strains was positive for CarbAcineto NP experiment,31 of 80 in A. baumannii strains was susceptible to imipenem and meropenem.PCR showed that OXA-23 gene was negative in 29 of 31 and CarbAcineto NP was negative in 30 of 31 non-carbapenemase-producing A.baumannii.The sen-sitivity and the specificity of CarbAcineto NP was 87.8% and 96.7%,respectively.Conclusion CarbAcineto NP was concordance with results obtained by PCR to detect gene coding for OXA-23.The CarbAcineto NP can detect rapidly carbapenemase producing in acinetobacter baumannii关键词
鲍曼不动杆菌/碳青霉烯酶/OXA-23/CarbAcinetoNPKey words
acinetobacter baumannii/carbapenemase/OXA-23 gene/CarbAcineto NP method分类
医药卫生引用本文复制引用
牛翠,黄红革,马芳,薛云,董月稳,时东彦..CarbAcineto NP快速检测耐碳青霉烯酶的鲍曼不动杆菌的方法学评价[J].检验医学与临床,2018,15(10):1428-1430,3.
