检验医学与临床2018，Vol.15Issue(10)：1434-1437,4.DOI:10.3969/j.issn.1672-9455.2018.10.019
胰岛素样生长因子结合蛋白相关蛋白1表达对鼻咽癌细胞系C N E1发生发展的研究
Inhibitory effect of IGFBP-rP1 expression on proliferation,invasion and migration of nasopharyngeal carcinoma cell line CNE 1
郑富春 1祁晓倩2
作者信息
- 1. 陕西省安康市人民医院耳鼻咽喉科 725000
- 2. 陕西省安康市人民医院检验科 725000
- 折叠
摘要
Abstract
Objective To investigate the inhibitory effect of insulin-like growth factor binding protein relat-ed protein 1(IGFBP-rP1)on proliferation,invasion and migration of nasopharyngeal carcinoma cell line CNE1.Methods The construction of two small interfering RNA specific for IGFBP-rP1 gene(siRNA32,siR-NA34)and negative control siRNA,CNE1 cells were divided into four groups:siRNA32 group(transfected with siRNA32 transfection),siRNA34 group(transfected with siRNA34),negative control group(negative control siRNA transfection)and blank group(no treatment).After training 24 h or 48 h,the related indexes were detected.Results The expression of IGFBP-rP1 mRNA and protein in siRNA32 group and siRNA34 group was significantly lower than that in negative control group and blank group(P<0.05).The number of CNE1 cells in siRNA32 group and siRNA34 group was higher than that in PVE-F membrane(P<0.05).The OD value of CNE1 cells in siRNA32 group and siRNA34 group was significantly lower than that in negative control group and blank group(P< 0.05).The OD value of CNE1 cells in siRNA32 group and siRNA34 group was significantly lower than that in negative control group and blank group(P<0.05).The percentage of G0/G1phase and G2/M phase cells in siRNA32 group and siRNA34 group was significantly higher than that in negative control group and blank group(P<0.05)was lower than the negative control group and the blank group(P<0.05).Conclusion Inhibition of IGFBP-rP1 expression can reduce the proliferation,invasion and migration of nasopharyngeal carcinoma CNE1 cells,and provide a new idea for the clinical treatment of naso-pharyngeal carcinoma.关键词
胰岛素样生长因子结合蛋白相关蛋白1/鼻咽癌/CNE1细胞Key words
insulin-like grow th factor binding protein-associated protein 1/nasopharyngeal carcinoma/CNE1 cells分类
医药卫生引用本文复制引用
郑富春,祁晓倩..胰岛素样生长因子结合蛋白相关蛋白1表达对鼻咽癌细胞系C N E1发生发展的研究[J].检验医学与临床,2018,15(10):1434-1437,4.
