检验医学与临床2018，Vol.15Issue(10)：1441-1444,4.DOI:10.3969/j.issn.1672-9455.2018.10.021
miR-135a与前列腺癌细胞增殖相关因子信号转导及转录激活因子6的相关性研究
The correlation between miR-135a and prostate cancer cell proliferation related factor STAT 6
赵宇明 1张悦 1王华 1田宝 1郭冬梅1
作者信息
摘要
Abstract
Objective To investigate the relationship between miR-135a and signal transducer and activator of transcription-6(STAT6)of prostate cancer cell proliferation related factors.Methods Prostate cancer DU145 cells were divided into miR-135a transfection group and blank transfection group randomly,miR-135a transfection group cells were transfected with miR-135a sequence,and blank transfection group was transfect-ed into blank sequence,cell proliferation was detected by MTT assay,and cell cycle was detected by flow cy-tometry;Transwell cell migration and invasion assay was used to detect the migration and invasion ability of cells,and the expression of p-STAT6 and STAT6 protein was detected by Western blot,miR-135a expression was detected by RT-PCR.Results After transfection 48 h,the expression of miR-135a in miR-135a transfec-tion group was(0.932 ± 0.061),which was significantly higher than that in the blank transfection group(P<0.05);miR-135a transfection group cultured 24 h and 48 h OD values were(0.210 ± 0.045)and(0.281 ± 0.052),significantly lower than the blank transfection group(P<0.05);The percentage of G0/G1cells in the miR-135a transfection group was(60.02 ± 6.39)%,which was significantly higher than that in the blank transfection group(P<0.05);the number of transfected cells and the number of invasive cells in miR-135a transfection group were(116.93 ± 30.02)and(133.02 ± 28.51),significantly lower than those in blank trans-fection group(P<0.05);the relative expression of p-STAT6 and STAT6 protein in miR-135a transfection group were(0.947 ± 0.114)and(0.437 ± 0.077),which was significantly lower than that in the blank trans-fection group(P<0.05).Conclusion MiR-135a can inhibit the proliferation,migration and invasion of pros-tate cancer cells,which may be related to its inhibition of STAT 6 expression in prostate cancer cells.关键词
前列腺癌/miR-135a/信号转导及转录激活因子6/转染Key words
prostate cancer/miR-135a/signal transducer and activator of transcription 6/transfec-tion分类
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赵宇明,张悦,王华,田宝,郭冬梅..miR-135a与前列腺癌细胞增殖相关因子信号转导及转录激活因子6的相关性研究[J].检验医学与临床,2018,15(10):1441-1444,4.
