检验医学与临床2018，Vol.15Issue(10)：1445-1447,3.DOI:10.3969/j.issn.1672-9455.2018.10.022
特应性皮炎患者外周血滤泡性辅助性T细胞的特征
Characteristics of follicular helper T cells in peripheral blood of patients with atopic dermatitis
刘芳 1陈端2
作者信息
- 1. 湖北省黄石市结核病防治院 435000
- 2. 湖北省黄石市临床检验中心 435000
- 折叠
摘要
Abstract
Objective To investigate the characteristics of follicular helper T(Tfh)cells in peripheral blood of patients with atopic dermatitis(AD),and to assess their roles in the immune pathogenesis of atopic dermatitis.Methods A total of 20 patients with atopic dermatitis and 12 health controls were enrolled in our study.The severity of atopic dermatitis was assessed by Scoring Atopic Dermatitis Index(SCORAD).All the patients were treated by Chinese traditional medicine and tacrolimus ointment.The Tfh cells frequency and ex-pressions of programmed death 1(PD-1),inducible costimulatory molecule(IOCS)on Tfh cells in peripheral blood of healthy normals,AD patients before and after 1-month treatment were measured by flow cytometry. The frequency of Tfh cells and expressions of PD-1,IOCS on Tfh cells between patients and health controls were analyzed,and the changes of those detective markers in AD patients before and after 1-month treatment were invested as well.Results Compared with health controls,the frequency of Tfh cells in AD patients was significantly increased(P<0.05).In addition,the expressions of PD-1 and IOCS on Tfh cells were significant-ly higher than them in health individuals as well(P<0.05).The frequency of Tfh cells in AD patients was positively correlated with SCORAD score(P<0.05),while the expressions of PD-1 and IOCS on Tfh cells did not correlated with SCORAD score.Furthermore,the Tfh cells frequency and the expressions of PD-1 and IOCS on Tfh cells were dramatically decreased after 1-month treatment(P<0.05).Conclusion The frequen-cy and function of Tfh cells were changed in AD patients,and Tfh cells may play important roles in the im-mune mechanism of atopic dermatitis.关键词
特应性皮炎/滤泡性辅助性T细胞/程序性死亡受体-1/诱导性共刺激分子Key words
atopic dermatitis/follicular helper T cells/PD-1/ICOS分类
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刘芳,陈端..特应性皮炎患者外周血滤泡性辅助性T细胞的特征[J].检验医学与临床,2018,15(10):1445-1447,3.
