临床肝胆病杂志2018，Vol.34Issue(5)：1075-1079,5.DOI:10.3969/j.issn.1001-5256.2018.05.029
谷氨酰胺在大鼠门静脉高压性胃病中的作用机制
Mechanism of action of glutamine in portal hypertensive gastropathy in rats
摘要
Abstract
Objective To investigate the role and mechanism of action of glutamine in portal hypertension gastropathy (PHG) in rats.Methods A total of 60 Sprague-Dawley rats were randomly divided into groups A (sham-operation),B (sham-operation + glutamine),C (partial portal vein ligation),and D (partial portal vein ligation + glutamine).A rat model of PHG was established by partial ligation of the main portal vein.Portal vein pressure (PVP),gastric injury index (GI),pathological integral (PI) of the gastric mucosa,and liver morphological changes were measured after 2 weeks,and the plasma levels of alanine aminotransferase (ALT),aspartate aminotransferase (AST),albumin (Alb),nitric oxide (NO),tumor necrosis factor-α (TNFα),and superoxide dismutase (SOD) were also measured.The t-test was used for comparison of continuous data between two groups,an analysis of variance was used for comparison between multiple groups,and the least significant difference t-test was used for further comparison between two groups.Results There was a significant difference in PVP between groups A,B,C,and D (1.17 ±0.15 kPa vs 1.21 ±0.19 kPa vs 2.65 ±0.16 kPa vs 2.18 ±0.22 kPa,F =4.60,P ＜0.05),and there were also significant differences between groups C/D and groups A/B (P ＜0.05).There were significant differences between groups C and D in GI (15.52 ± 2.05 vs 8.26 ± 1.23,t =5.84,P ＜ 0.05) and PI (7.56 ± 1.53 vs 3.15 ± 1.42,t =7.45,P ＜ 0.05).There were no significant differences in ALT,AST,and Alb between the four groups (all P ＞ 0.05).There were significant differences between the four groups in TNFα (0.56 ± 0.11 ng/ml vs 0.41 ± 0.21 ng/ml vs 1.35 ± 0.26 ng/ml vs 0.68 ± 0.21ng/ml,F =5.24,P ＜ 0.05),NO (1.63 ± 0.15 μmol/gpro vs 1.41 ± 0.12 μmol/gpro vs 5.52 ± 1.06 μmol/gpro vs 2.26 ± 0.83 μmol/gpro,F =8.40,P ＜0.05),and SOD (148.21 ± 6.21 U/mg vs 154.21 ± 5.31 U/mg vs 74.56 ± 4.21 U/mg vs 135.25 ± 4.82U/mg,F =14.78,P ＜ 0.05).There were significant differences in TNFα,NO,and SOD between group C and groups A/B (all P ＜0.05),as well as between groups C and D (all P ＜ 0.05).Conclusion PHG has various pathogenic factors.Glutamine can alleviate gastric mucosal edema and hemorrhage in rats with PHG and reduce the content of NO,TNFα,and SOD.Glutamine has a good antioxidant effect and thus helps with the treatment of PHG.关键词
门静脉高压性胃病/谷氨酰胺/大鼠,Sprague-DawleyKey words
portal hypertensive gastropathy/glutamine/rats, sprague-dawley分类
医药卫生引用本文复制引用
黄湘俊,王永恒,段珊珊,李澜,孙国辉,杨媚月,冷大跃,张文兴..谷氨酰胺在大鼠门静脉高压性胃病中的作用机制[J].临床肝胆病杂志,2018,34(5):1075-1079,5.基金项目
湖南省科技厅项目(2013FJ4058) （2013FJ4058）
湖南省中医药管理局项目(201355) （201355）
