环境与职业医学2018，Vol.35Issue(4)：297-302,6.DOI:10.13213/j.cnki.jeom.2018.17694
氟、砷染毒对大鼠破骨细胞骨吸收能力的影响及其机制
Effects of fluoride and arsenic exposure on bone absorptive capacity of osteoclasts in rats and related mechanism
摘要
Abstract
[Objective] To explore the toxicity and mechanism of combined exposure to fluoride and arsenic on bone absorptive capacity of osteoclasts in rats.[Methods] A total of 54 SD rats of clean grade were randomly divided into 9 groups,with 6 rats in each group (half males and half females).Based on a pilot experiment and literature review,the animals were treated with NaF and NaAsO2 by intragastric administration:control group (FoAso),low fluoride group (5 mg/kg,F5),high fluoride group (20 mg/kg,F20),low arsenic group (2.5 mg/kg,As2.5),high arsenic group (10 mg/kg,As10),low fluoride and low arsenic group (5 mg/kg+2.5 mg/kg,F5As2s),low fluoride and high arsenic group (5 mg/kg+10mg/kg,F5As10),high fluoride and low arsenic group (20mg/kg+2.5 mg/kg,F20Asz5),and high fluoride and high arsenic group (20mg/kg+10mg/kg,F20As10).The levels of tartrateresistant acid phosphatase 5b (TRACP-5b) and pyridinoline cross linked carboxyterminal telopeptide of type Ⅰ collagen (ICTP) in rat serum,as well as the levels of osteoprotegerin (OPG),prostaglandin (PGE2),and 1,25-dihydroxy vitamin D3 [1,25(OH)2D3] in homogenate supematant of fight femur tissue samples were determined by ELISA.[Results] The expression levels of ICTP and TRACP-5b in the high fluoride group were higher than those in the control group (FoAs0:1.352 ± 0.025,2.942 ± 0.078;F20:1.427 ± 0.026,3.202 ± 0.142;Ps ＜ 0.05),while there was no significant difference between any individual arsenic exposure group and the control group (Ps ＞ 0.05).The expression levels of ICTP and TRACP-5b in the high fluoride and high arsenic group were lower than those in the high fluoride and low arsenic group (F20As2.5:1.442 ± 0.034,3.280 ± 0.095;F2oAs10:1.345 ± 0.042,3.053 ± 0.084;Ps ＜ 0.05).The results of partial correlation analysis and interaction analysis showed that the expression levels of TRACP-5b and ICTP were positively correlated with fluoride concentration (partial correlation coefficient:0.663,0.392;Ps ＜ 0.05),and fluoride showed the main effect (F=24.560,8.188;Ps ＜ 0.05).The expression levels of OPG,PGE2,and 1,25(OH)2D3 in the groups treated with high doses of fluoride were significantly higher than those in the control group (FoAs0:12.278 ± 0.220,1.352 ± 0.025,9.582 ± 0.363;F20:14.087 ± 0.797,1.427 ± 0.026,11.915 ± 0.931;Ps ＜0.05).The expression level of PGE2 in the low fluoride and high arsenic group was lower than that in the low fluoride and low arsenic group (F5As2.5:1.442 ± 0.034;F5As10:1.345 ± 0.080;P＜0.05).The expression level of 1,25(OH)2D3 in the high fluoride and high arsenic group was lower than that in the high fluoride and low arsenic group (F20As2.5:12.082 ± 0.860;F2oAs1o:10.567 ± 0.695;Ps ＜ 0.05).The results of partial correlation analysis and interaction analysis showed that the expression levels of OPG,PGE2,and 1,25(OH)2D3 were positively correlated with fluoride concentration (partial correlation coefficient:0.804,0.487,0.748;Ps ＜ 0.05),and fluoride showed as the main effect (F=47.100,9.031,3.114;Ps ＜ 0.05);the direct toxic effects of arsenic on OPG,PGE2,and 1,25(OH)2D3 were minor (P＞ 0.05).[Conclusion] Arsenic can inhibit the anxo-action of fluorine on the bone absorption of osteoclasts in rats,and the fluorine-arsenic interaction is mainly antagonistic.PGE2 and 1,25(OH)2D3 may be important factors in the regulation of osteoclast bone absorption function.关键词
氟/砷/骨/联合作用/破骨细胞Key words
fluoride/arsenic/bone/combined effect/osteoclast分类
医药卫生引用本文复制引用
杨德山,洪峰..氟、砷染毒对大鼠破骨细胞骨吸收能力的影响及其机制[J].环境与职业医学,2018,35(4):297-302,6.基金项目
国家自然科学基金项目(编号:81472927) （编号:81472927）
