环境与职业医学2018，Vol.35Issue(4)：356-360,5.DOI:10.13213/j.cnki.jeom.2018.17604
镉通过类雌激素样作用促进人乳腺癌MCF-7细胞增殖
Estrogen-like effect of cadmium on promoting proliferation of human breast cancer MCF-7 cells
摘要
Abstract
[Objective] To investigate the effect and potential mechanism of cadmium (Cd) on the proliferation of human breast cancer MCF-7 cells and the expression of estrogen receptor (ER).[Methods] A and B strains of MCF-7 cells were treated with 17β-estradiol (hereinafter referred to as estrogen) at 1 × 10-6,1 × 10-7,1 × 10-8,1 × 10-9,1 × 10-10,and 1 × 10-11 mol/L,respectively,for 4d.CCK8 assay was employed to detect a susceptible MCF-7 cell strain and to identify the concentration of estrogen promoting maximum cell proliferation.Then,the susceptible MCF-7 cell strain was treated with 1 × 10-6,1 × 10-7,1 × 10-8,1 × 10-9,1 × 10-10,and 1 × 10-11 mol/L cadmium solution,respectively,to identify the concentration of cadmium promoting maximum cell proliferation.We set a negative control group (medium without estrogen),an experimental group (1 × 10 mol/L cadmium),and a positive control group (1 × 10-9mol/L estrogen) to evaluate colony formation ability by clonogenicity assay.Additionally,we set an experimental inhibitor group [1 × 10-8 mol/L Cd+1 × 10-7 mol/L ER antagonist (ICI 182780)] and a positive inhibitor group (1 × 10-9 mol/L estrogen+ 1 × 10-7 mol/L ER antagonist) to detect cell proliferation,the ratio of S phase cells,and the expression level of ER by CCK8,flow cytometry,and Western blot,respectively.[Results] The MCF-7 cells of strain B were estrogen sensitive cells,and the 1 × 10-9 mol/L estrogen treatment induced the maximum cell proliferation [(203.55 ± 36.65)％].Compared with the negative control group (100％),1 × 10-8-1 × 10-10 mol/L cadmium significantly promoted proliferation of MCF-7 cells [(163.78 ± 31.90)％-(176.88 ± 10.06)％] (P ＜ 0.001).Besides,1 × 10-8 mol/L cadmium treatment promoted colony formation of MCF-7 cells (P ＜ 0.05),increased the ratio of S phase cells (P ＜ 0.001),and elevated ER protein expression level (P＜0.001).Compare with the experimental group,ER antagonist suppressed the effect of cadmiium on proliferation of MCF-7 cells [from (135.17 ± 23.96)％ to (107.66 ± 7.64)％] (P＜0.01),reduced the ratio of S phase cells [from (24.17 ± 0.53)％ to (12.36 ± 0.43)％] (P ＜ 0.001),and down-regulated ER protein expression level [from (56.19 ± 3.67)％ to (38.84 ± 1.04)％] (P＜0.05).[Conclusion] Cadmium can promote proliferation and ER expression of breast cancer MCF-7 cells,and the effect can be inhibited by ER antagonist,indicating estrogen-like effect of cadmium.关键词
镉/乳腺癌细胞/17β-雌二醇/类雌激素样作用/雌激素受体Key words
cadmium/human breast cancer cell/17β-estradiol/estrogen-like effect/estrogen receptor分类
医药卫生引用本文复制引用
凌晓斐,胥可,孙亚昕,邵华,王翠娟..镉通过类雌激素样作用促进人乳腺癌MCF-7细胞增殖[J].环境与职业医学,2018,35(4):356-360,5.基金项目
山东省医学科学院医药卫生科技创新工程(编号:2015WS0167) （编号:2015WS0167）
山东省医药卫生科技发展计划项目(无编号) （无编号）
