环境与职业医学2018，Vol.35Issue(4)：361-365,5.DOI:10.13213/j.cnki.jeom.2018.17645
CHIP和Hsp70在三氯化铝致N2a细胞tau蛋白异常磷酸化中的作用
Effects of CHIP and Hsp70 on abnormal phosphorylation of tau protein induced by aluminum trichloride in N2a cells
摘要
Abstract
[Objective] To investigate the effects of carboxyl terminus of Hsc70-interacting protein (CHIP) and hot shock protein 70 (Hsp70) on aluminum trichloride induced abnormal phospborylation of tau protein in mouse neuroblastoma N2a cells.[Methods] N2a cells were cultured with low,middle,and high doses of aluminium trichloride (0.5,1.0,and 2.0 mmol/L,respectively),and the control group was treated without aluminium trichloride.The cell morphological changes were observed after 48 h of exposure under microscope and the cell viability was measured by CCK-8 assay.The protein expression levels of tau-5,pThrl81,pThr231,pSer262,pSer396,CHIP,and Hsp70 were measured by Western blot.[Results] As the aluminum trichloride concentration increased,the count of N2a cells decreased,the synapses retracted,and the cell body rounded.The middle and high dose groups showed significantly lower cell viabilities [(91.37 ± 0.03)％ and (78.45 ± 0.10)％] than the control group [(100.00 ± 0.00)％] (P ＜ 0.05,P ＜ 0.01).The expression level of tau-5 protein of the high dose group was significantly higher than that of the control group (P＜ 0.01);the expression levels of pThr231 of the middle and high dose groups were significantly higher than that of the control group (P ＜ 0.01);the expression levels of pSer396 were higher in all aluminum trichloride groups than in the control group (P＜0.05 or P＜0.01);but no differences were found in the expression levels of pThr181 and pSer262 (P＞ 0.05).The expression levels of CHIP and Hsp70 of all aluminium trichloride groups were significantly higher than those of the control group (P＜ 0.05 or P＜ 0.01).[Conclusion] Aluminum trichloride could increase the expression levels of pThr231 and pSer396 in N2a cells,which is possibly related to the expression changes of CHIP and Hsp70.关键词
CHIP/Hsp70/铝/tau蛋白/磷酸化Key words
CHIP/Hsp70/aluminum/tau protein/phosphorylation分类
医药卫生引用本文复制引用
崔双杰,巨晓芬,潘宝龙,张玲,路小婷..CHIP和Hsp70在三氯化铝致N2a细胞tau蛋白异常磷酸化中的作用[J].环境与职业医学,2018,35(4):361-365,5.基金项目
国家自然科学基金(编号:81001241,81472959) （编号:81001241,81472959）
