老年医学与保健2018，Vol.24Issue(2)：149-153,5.
活性氧簇调控细颗粒物诱导人支气管上皮细胞凋亡的机制研究
Mechanism of ROS Regulating PM2.5-induced Apoptosis in Human Bronchial Epithelial Cells
摘要
Abstract
Objective To investigate the mechanism of reactive oxidative species (ROS) regulating the apoptosis of human bronchial epithelial cells (HBE) induced by PM2.5.Methods HBE cells were stimulated with 0,100,200 and400 μg / mL PM2.5 for 24 h respectively,then the activity was detected by CCK-8,the generation of ROS in cells was detected by DCFH-DA fluorescent labeling,the apoptosis rate was detected by Annexin V-Alexa Fluor 488 / PI double-labeled flow cytometry,the apoptosis-related proteins expression of Bax,Bcl-2 and Caspase-3 were detected by Western blot.Results The results of CCK-8 assay showed that PM25 significantly inhibited the activity of HBE,induced the generation of ROS in HBEcellsandapoptosis,it also increased the expression ofBax,Caspase-3,and decrease the expression ofBcl-2;in addition,inhibition of ROS production with N-acetyl-L-cysteine (NAC) attenuated PM2 2-induced inhibition of HBE activity and apoptosis,suggesting that PM25-induced apoptosis was associated with ROS.Conclusions PM25 can induce the generation of ROS in HBE cells and then induce the apoptosis,which may be one of the mechanisms that affect the respiratory system function.关键词
细颗粒物/支气管上皮细胞/活性氧簇/细胞凋亡Key words
PM2.5/bronchial epithelial cells/reactive oxidative species (ROS)/apoptosis引用本文复制引用
郑洋,葛海燕,潘林约,褚国坤,赵文文,朱惠莉..活性氧簇调控细颗粒物诱导人支气管上皮细胞凋亡的机制研究[J].老年医学与保健,2018,24(2):149-153,5.基金项目
国家自然科学基金青年资助项目(81600056) （81600056）
上海市市级医院三年行动计划项目(16CR4003A) （16CR4003A）
