热带作物学报2017，Vol.38Issue(12)：2285-2293,9.DOI:10.3969/j.issn.1000-2561.2017.12.015
橡胶树花青素合成调控因子HbAn1的克隆及其功能分析
Cloning and Function Analysis of R2R3-MYB Regulatory Factor for Anthocyanin Biosynthesis in Hevea brasiliensis
摘要
Abstract
The low screening efficiency for the positive embryo of rubber trees hinders the development of transgenic research in Hevea.Red purple from anthocyanin,which can be detected by naked eyes,is a simple and safe visual marker.In order to take anthocyanin as the selective marker to improve the screening efficiency for the positive embryo of rubber trees,R2R3-MYB of rubber trees was cloned based on the conserved domain of R2R3-MYB,and the phylogenetic relationship with the anthocyanin-related R2R3-MYB of Arabidopsis thaliana and other species was analyzed,Results showed that scaffold 0374_923317 and Scaffold 0598_393375 were classified in the same sub-group with other known anthocyanin-related R2R3-MYB regulators.Then scaffold0374_923317 isolated from the bronze leaf,and named as HbAn1,had the typical conserved R2R3 and bHLH interaction domain.Quantitative expression analysis revealed that the expression of HbAn1 decreased gradually with the development of rubber leaves,which was coincided with the anthocyanin accumulation contents of rubber leaves.The expression of HbA n1 was also coincided with green and red stems,indicating that the expression of HbAn1 was positively correlated with rubber tree anthocyanin accumulation.Finally,in constitutive overexpression HbAn1 tobacco,the anthocyanin accumulation increased extremely in tobacco leaves,petals,filaments and receptacles caused by activating the late flavonoid pathway genes NtDFR,NtLDOX,NtUFGT.In this paper,for the first time,R2R3-MYB positively regulating anthocyanin accumulation in H.brasiliensis was isolated,which would provide a gene resource for the visual selection marker of H.brasiliensis transformation.关键词
橡胶树/花青素/可视化筛选标记/R2R3-MYB/结构域Key words
Hevea brasiliensis/anthocyanin/visual selection marker/R2R3-MYB/domain分类
农业科技引用本文复制引用
黄天带,方永军,畅姣,陈涛,辛士超,Naychi Koko,黄华孙,华玉伟..橡胶树花青素合成调控因子HbAn1的克隆及其功能分析[J].热带作物学报,2017,38(12):2285-2293,9.基金项目
海南省重点研发计划科技合作方向项目(No.ZDYF2016220) （No.ZDYF2016220）
中国热带农业科学院橡胶研究所基本科研业务费专项(No.1630022017020) （No.1630022017020）
中国热带农业科学院基本科研业务费专项资金(No.1630022017015). （No.1630022017015）
