热带作物学报2017，Vol.38Issue(12)：2302-2309,8.DOI:10.3969/j.issn.1000-2561.2017.12.017
香樟MK基因的克隆与生物信息学分析
Cloning and Bioinformatic Analysis of MK Gene from Cinnamomum camphora
摘要
Abstract
Based on the transcriptome data of Cinnamomum camphora,specific primers were designed by reverse transcription RT-PCR to clone the full-length ORF sequence of Mevalonate kinase (MK) from C.camphora (Cc).According to the method of homologous recombination of Gibison gene CcMK gene was cloned into the pSMART-LCKan vector.The recombinant plasmid was identified with resistance and DNA sequencing.Bioinformatics analysis on CcMK was carried out.Sequencing results showed that the CcMK gene ORF was 1 194 bp,encoding 397 amino acids.Bioinformatic analysis showed that the molecular formula of CcMK protein was C1852H3027N487O574S17,The relative molecular mass was 41 845.34,and the isoelectric point was 5.30.CcMK protein was a stable protein localized in cytoplasm with no signal peptide,which contained a GHMP kinase family specific N terminal and a conserved domain of C terminal.The molecular phylogenetic tree analysis showed that CcMK protein and Anthurium amnicola,Fritillaria cirrhosa,musa acuminata subsp malaccensis,Phoenix dactylifera,Elaeis guineensis MK protein were in the same branch of close relationship.CcMK gene was cloned firstly from C.camphora and analyzed by bioinformatics.These results would lay a foundation for further research on the role of CcMK gene in the terpene synthesis of C.camphora.关键词
香樟/甲羟戊酸激酶/基因克隆/生物信息学分析Key words
Cinnamomum camphora/Hydroxy acid kinase/gene cloning/bioinformatics analysis分类
农业科技引用本文复制引用
曹先爽,王进,张瑶瑶,王煜炜,马晓江,宋丽,汤锋,岳永德..香樟MK基因的克隆与生物信息学分析[J].热带作物学报,2017,38(12):2302-2309,8.基金项目
国家林业公益性行业科研专项重点项目(No.201404601) （No.201404601）
国家林业局948项目(No.2014-4-33). （No.2014-4-33）
