热带作物学报2017，Vol.38Issue(12)：2394-2399,6.DOI:10.3969/j.issn.1000-2561.2017.12.030
芭蕉植株上乳酸菌的分离鉴定及抑菌菌株的初步筛选
The Separation and Identification of Lactobacilli from Plantain and the Initial Screening of Bacteriostatic Strains
摘要
Abstract
This study was aimed to analyse the microbial flora of Musa basioo Siebold,to separate and breed lactobacilli strains,as well as to expand the culture collection of the lactobacilli strains.Colony-counting method was used to count the number of lactobacilli,aerobic bacteria,mycetes,saccharomycetes and bacilli in the samples.Physiological and biochemical characteristics,growth rate and acid production rate of the suspected lactobacilli were analysed,therefore the genera of the suspected lactobacilli were determined.Bacteriostatic experiments were conducted on Escherichia coli,Salmonella and Listeria monocytogenes.Results showed that the sample contained aerobic bacteria (6.5～8.0 log cfu/g FM),mycetes (6.0～7.7 log cfu/g FM),saccharomycetes (2.0～3.3 log cfu/g FM),bacilli (2.0～4.4 log cfu/g FM) and lactobacilli (6.5～7.5 log cfu/g FM).34 suspected lactobacilli strains identified as lactobacilli were screened out from the plantain plates.Among the 40 suspected lactobacilli,four were plant lactobacillus.关键词
芭蕉/乳酸菌/分离鉴定/抑菌Key words
Plantain/lactobacilli/separation and identification/bacteriostasis分类
生物科学引用本文复制引用
郗恩光,谭海生,杨劲松,崔坤鹏,张万昌,鞠雪莉,李晓雷,张桂和..芭蕉植株上乳酸菌的分离鉴定及抑菌菌株的初步筛选[J].热带作物学报,2017,38(12):2394-2399,6.基金项目
国家自然科学基金资助项目(No.31460621). （No.31460621）
