热带作物学报2018，Vol.39Issue(1)：46-54,9.DOI:10.3969/j.issn.1000-2561.2018.01.008
基于EST-SSR毛细管电泳荧光标记技术分析广东2个历史名茶群体遗传多样性
Genetic Diversity and Relationship of Two Historical Famous Camellia Sinensis Groups in Guangdong by Capillary Electrophoresis Detcection with Fluorescent EST-SSR Marker
摘要
Abstract
EST-SSR capillary electrophoresis fluorescence maker technique was used to analyze the genetic polymorphism and genetic relationship of 52 tea plant germplasm resources from two historical famous Camellia Sinensis groups of Luokeng and MaTu in Guangdong.In total,204 alleles were detected by 27 EST-SSR primers,with an average of 7.56 locus per primer,and the genotypes was 11.22.The numbers of observed alleles and effective alleles was 7.56 and 3.42.The observed heterozygosity (Ho) and expected heterozygosity (He) was 0.55 and 0.66,respectively.The average of polymorphism information content (PIC) was 0.61,and the Shannon index of the 52 germplasms was 1.40.The Nm (gene flow) of the two groups was 3.19,showing gene exchange might occur in the past time between the two populations.And Fst was 0.0727.The genetic diversity of the two groups was similar.The 52 tea plant germplasms were divided into three subgroups containing 18,24,9 tea plant germplasms resources,respectively,based on statistical model of Evanno egc.,and the germplasm of MT4 did not belong to any subgroups.关键词
茶树种质资源/遗传多样性/群体结构/毛细管电泳Key words
tea germplasms/genetic diversity/population structure/capillary electrophoresis分类
生物科学引用本文复制引用
姜晓辉,方开星,陈栋,吴华玲..基于EST-SSR毛细管电泳荧光标记技术分析广东2个历史名茶群体遗传多样性[J].热带作物学报,2018,39(1):46-54,9.基金项目
现代农业产业(茶叶)技术体系专项(No.CARS-23) （茶叶）
广东省科技计划项目(No.2015A030302047,No.2013B020305004,No.2016A090924005,No.2014A030305011,No.2015B090906027) （No.2015A030302047,No.2013B020305004,No.2016A090924005,No.2014A030305011,No.2015B090906027）
广东大叶种红茶产业技术创新平台建设. （）
