热带作物学报2018，Vol.39Issue(1)：93-99,7.DOI:10.3969/j.issn.1000-2561.2018.01.015
不同种皮颜色花生品种花青素合成相关基因的克隆及序列差异分析
Clone and Sequence Analysis of Genes Associated with Anthocyanin Synthesis in Peanut Cultivars with Different Testa Color
摘要
Abstract
The color of testa (seed coat) is associated with species and accumulation of anthocyanin.The genes relative to anthocyanin accumulation,including PAL,CHI,CHS,DFR,F3'H,3GT and ANS were isolated using RACE kits from 4 peanut cultivars with different testa colors.Sequences in open reading frame of these genes were compared among 4 peanut cultivars with different testa color.The results showed that there were respectively 8,1,1,12,1 and 9 amino acid transition sites in CHS,CHI,DFR,F3'H,ANS and 3GT among the 4 peanut cuhivars.All the sites of different amino acid transition was not located in conservatized zones.Whether the structural difference of these genes is related to the color difference of peanut seed coat remains to be further explored.关键词
花生/种皮颜色/花青素/氨基酸替换Key words
peanut/color of testa/anthocyanin/amino acid transition分类
农业科技引用本文复制引用
李海芬,邱金梅,陈小平,梁炫强..不同种皮颜色花生品种花青素合成相关基因的克隆及序列差异分析[J].热带作物学报,2018,39(1):93-99,7.基金项目
国家自然科学基金青年基金“花生荚果果型大小相关基因的筛选与分析”(No.31501246) （No.31501246）
广东省科技计划项目:“高油酸花生新品种培育及产业化应用研究”(No.2015B020231006) （No.2015B020231006）
“黑花生种质资源的创制和新品种的培育”(No.2015A020209051) （No.2015A020209051）
“花生高油酸材料的创制及新品种的培育研究任务”(No.2016LM3161). （No.2016LM3161）
