热带作物学报2018，Vol.39Issue(1)：108-115,8.DOI:10.3969/j.issn.1000-2561.2018.01.017
基于SLAF-seq技术的苦瓜白粉病SNP分子标记开发
Development of SNP Molecular Markers for Powdery Mildew in Bitter Gourd Using SLAF-seq Technique
摘要
Abstract
MC18 (highly powdery mildew-resistant),MC105 (highly powdery mildew-sensitive) and the F2 segregating population were used as the experimental materials for sequencing by specific-locus amplified fragment sequencing (SLAF-seq),a high-throughput reduced-representation genotyping technology.In this study,a total of 10.48 Mb reads were obtained,and 91856 high quality SLAFs were produced,of which 5502 were polymorphisms.Finally,we developed 29 specific fragments of powdery mildew in bitter gourd using SLAF-seq technique,and Tetra-primer ARMS-PCR technology was used to validate SNP genetic typing of bitter gourd,and Marker4542,Marker11188 molecular markers detected could be closely linked to the genes corresponding to the target traits.The results would lay a good foundation for bitter gourd MAS resistance breeding.关键词
苦瓜/白粉病/SLAF-seq/SNP分子标记/ARMS-PCRKey words
bitter gourd/powdery mildew/SLAF-seq/SNP molecular marker/ARMS-PCR分类
农业科技引用本文复制引用
冯诚诚,黄如葵,黄熊娟,陈小凤,黄玉辉,梁家作,刘杏连,王齐旭..基于SLAF-seq技术的苦瓜白粉病SNP分子标记开发[J].热带作物学报,2018,39(1):108-115,8.基金项目
现代农业产业技术体系专项项目(No.CARS-25) （No.CARS-25）
国家自然科学基金(No.31760580) （No.31760580）
广西科学研究与技术开发计划项目(桂科合15104003-1-1 （）
桂科AB16380059) （）
广西自然科学基金项目(No.2015GXNSFBA139079). （No.2015GXNSFBA139079）
