热带作物学报2018，Vol.39Issue(1)：129-136,8.DOI:10.3969/j.issn.1000-2561.2018.01.020
海南橡胶树炭疽菌Colletotrichum siamense和C.fructicola的鉴定及系统发育分析
Identification and Phylogenetic Analysis of Anthracnose Pathogen Colletotrichum siamense and C.fructicola Isolated from Rubber Tree in Hainan
摘要
Abstract
Colletotrichum gloeosporioides species complex are reported as the main pathogen of the rubber anthracnose,which is one of the important leaf diseases of rubber tree in China.But accurate identification of the causal organisms is not known till now.In this study,thirteen strains of C.gloeosporioides species complex from the rubber tree in Hainan were selected for further species accurate identification.Partial mating type (Mat1-2) gene (ApMAT) and glutamine synthetase (GS) of them were cloned and the molecular phylogenetic tree of them were analyzed comparatively based on the partial sequences of ApMAT single gene,2-gene combined dataset (GS,ApMAT) and 6-gene combined dataset (ITS,ACT,CHS-1,GAPDH,GS,ApMAT),respectively.The results showed that the phylogenetic trees of the 13 isolates belong to two groups of C.siamense and C.fructicola,no matter the trees were builded using ApMAT single gene or 2-gene combined dataset (GS,ApMAT) or 6-gene combined dataset.It indicated that the 13 isolates of C.gloeosporioides species complex from the rubber tree in Hainan were identified as C.siamense and C.fructicola.In addition,our study demonstrated that the combined use of ApMat and GS in a phylogenetic analysis can identify the species in the C.gloeosporioides species complex.关键词
橡胶树/胶孢炭疽菌复合群/多基因序列分析法/分子鉴定Key words
rubber tree/Colletotrichum gloeosporioides species complex/multiple-gene phylogeny/molecular identification分类
农业科技引用本文复制引用
林春花,杨欢,赵晓宇,潘潇涵,刘文波,缪卫国,郑服丛..海南橡胶树炭疽菌Colletotrichum siamense和C.fructicola的鉴定及系统发育分析[J].热带作物学报,2018,39(1):129-136,8.基金项目
现代农业产业技术体系建设专项资金项目(No.CARS-34-BC1) （No.CARS-34-BC1）
国家自然科学基金(No.31560495) （No.31560495）
“海南省高等学校教育教学研究项目(No.Hnjg2016ZD-2,No.hdjy1606) （No.Hnjg2016ZD-2,No.hdjy1606）
海南大学科研启动基金项目(No.kyqd1535). （No.kyqd1535）
