热带作物学报2018，Vol.39Issue(1)：137-144,8.DOI:10.3969/j.issn.1000-2561.2018.01.021
华石斛内生真菌组织分布特点及其抑菌活性
Diversity of Endophytic Fungi Isolated from Dendrobium sinense with Different Culture Media and Their Antimicrobial Activities
摘要
Abstract
Due to their species and functional diversity,plant endophytic fungi have gradually become a hot topic in current plant biotechnology.In this study,endophytic fungi were separated from an Hainan endemic medicinal plant Dendrobium sinense to explore their diversity,tissue distributing characteristics and antimicrobial funtions,in order to set a foundation for further invention of new agricultural fungicides.The results are presented as following:179 strains of endophytic fungi were isolated and identified as 51 OTUs in 25 taxons based on ITS sequences analysis.Among them,Phyllosticta was the dominant genera.Except Daldinia which widely distributed in roots,stems and leaves,all of the other taxons showed tissue bias.18 taxons only distributed in one kind of tissue.The number of strains isolated from leaves was accounted for the biggest isolation rate of 60.33％.The taxons of endophytic fungi isolated with different kinds of culture medium were varied.Among all of the five culture media,PDA and D.sinense natural medium had the best endophyte capture ability,with 12 taxons (48％ of the total taxon number) being isolated from both of them separately.Antimicrobial activity assay showed that 36 strains in 12 taxons were antagonistic to at least one kind of the testing fungi.One strain of Pestalotiopsis and one strain of Talaromyces showed the strongest antifungal ability.Above all,the diversity of D.Sinense endophytic fungi was very rich.The antifungal bioactivity of some endophyte strains were strong,and worth further study in the future.The results in this study set a foundation for in-depth explosion of endophytic resources of D.sinense.关键词
内生菌/药用石斛/多样性/抗菌性Key words
endophyte/medicinal dendrobium/diversity/antimicrobial activity分类
农业科技引用本文复制引用
柴晓蕾,宋希强,朱婕..华石斛内生真菌组织分布特点及其抑菌活性[J].热带作物学报,2018,39(1):137-144,8.基金项目
海南省自然科学基金面上项目(No.20163045) （No.20163045）
中央级公益性科研院所基本科研业务费专项资金资助项目(No.1630052016008) （No.1630052016008）
海南大学科研启动基金项目(No.kyqd1549) （No.kyqd1549）
2017年海南大学基础研究(青年基金)(No.hdkyxj201711). （青年基金）
