热带作物学报2018，Vol.39Issue(1)：145-150,6.DOI:10.3969/j.issn.1000-2561.2018.01.022
赤芝担孢子交配型因子鉴定及多样性分析
Determination and Diversity Analysis of Mating Types of Ganoderma lucidum Basidispores
摘要
Abstract
The diversity of mating types was analyzed by the protoplast monokaryons of 7 strains of Ganderma lucidum.The mating types of the basidispores of G.lucidum JC and AL were determined by the method of three-cycle mating system,OWE-SOJ technique and nuclear migration test.The results showed that at least 8 A and 8 B factors were found in the 7 test strains.It was found that the monokaryon segregation of AL,Han-zhi No.1,Song-shan and Gl.0032+2 was as expected by x2 test,while the different mating types of protoplasted monokaryon of Xue-zhi and Wu-bao,the basidispores of JC did not display the expected segregation ratio by x2 test.Four mating types of the basidispores of G.lucidum were determined by OWE-SOJ technique and the nuclear migration test,which verified each other.The monokaryons of JC:T1(AxBx),T2(AyBy),T3(AyBx) and T4 (AxBy).The monokaryons of AL:T1(AxBx),T2(AyBy),T3(AxBY) and T4(AyBx).关键词
赤芝/交配型/四级性/亲和Key words
Ganoderma lucidum/mating type/tetrapolarity/affinity分类
农业科技引用本文复制引用
柯斌榕,卢政辉,吴小平,郭丽羡,兰清秀..赤芝担孢子交配型因子鉴定及多样性分析[J].热带作物学报,2018,39(1):145-150,6.基金项目
福建省食用菌产业重大农技推广服务试点项目(灵芝、瓶栽银耳创新服务团队)(No.KNJ-153013) （灵芝、瓶栽银耳创新服务团队）
食用菌产业农技推广服务体系建设(No.KNJ-153012). （No.KNJ-153012）
