热带作物学报2018，Vol.39Issue(1)：174-181,8.DOI:10.3969/j.issn.1000-2561.2018.01.027
木奶果醇提物的抗氧化活性及其对Aβ25-35致PC12细胞损伤的神经保护作用
Antioxidant Activity of Ethanol Extracts of Baccaurea Fruit and Its Neuroprotective Effect on PC12 Cells Damage Induced by Aβ25-35
摘要
Abstract
The total phenolic contents,antioxidant activity and the neuroprotective effect on PC12 cells damage induced by Aβ25-35 of different parts of Baccaurea fruit (BF) were investigated to provide theoretical basis for developing high value products of BF.In this study,the ethonal extracts of different parts of BF (peel,pulp,kern) were subjected to the following assay.The total phenolic contents of the alcohol extracts were determined by Folin-Ciocalteu method,and the antioxidant activity of the ethonal extracts was evaluated by DPPH,ABTS and hydroxyl system.The neuroprotective effect was investigated by using Aβ25-35-induced damage in PC12 cells.The cell viability of PC12 cells was detected by CCK-8 assay and the apoptosis was detected by TUNEL reaction kit.The total phenolic contents of the ethonal extracts of the three parts were 101.03±5.99,16.03±1.13,51.27±4.02 mg gallic acid equivalents (GAE) per 1 g dried weight respectively.The antioxidant activity increased in a concentration dependent manner.The highest antioxidant activity and neuroprotection were observed in the peel.By adding 1 mg/mL of peel extracts,the lethality rate of PC12 cell decreased from (41.6+1.36)％ to (11.95+1.98)％,and the apoptosis rate of PC12 cell decreased from (84.69+5.78)％ to (25.26+3.18)％.In conclusion,the alcohol extracts of the BF has good neuroprotective effect on PC12 cells damage induced by Aβ25-35.关键词
木奶果/醇提物/抗氧化/神经保护Key words
baccaurea/ethanol extracts/antioxidant/neuroprotection分类
农业科技引用本文复制引用
郑晓燕,盛占武,谢学历,阙燕鹏,谭琳,艾斌凌,郑丽丽..木奶果醇提物的抗氧化活性及其对Aβ25-35致PC12细胞损伤的神经保护作用[J].热带作物学报,2018,39(1):174-181,8.基金项目
海南省自然科学基金(No.20163100). （No.20163100）
