热带作物学报2018，Vol.39Issue(1)：197-207,11.DOI:10.3969/j.issn.1000-2561.2018.01.030
CRISPR/Cas9系统在植物育种中的应用
Application Progress of CRISPR/Cas9 in Plant Breeding
摘要
Abstract
Artificial nuclease systems are powerful tools for gene editing and allow to create new alleles by digesting the target loci that introducing the locus-specific strand breaks and being addressed by endogenous repair pathways.CRISPR/Cas9 system is a natural adaptive immune system for resisting phage infection in prokaryotes.The advanced CRISPR/Cas9 editing system is superior to other nuclease systems such as zinc finger nuclease system and TALE nuclease systemby virtue of the characteristics of simple operation,high efficiency,low cost.At present,the optimized CRISPR/Cas9 editing system has been widely used to identify the function of plant gene and create new plant materials.In this paper,we reviewed the structure and mechanism of CRISPR/Cas9 editing system,the editing efficiency of CRISPR/Cas9 system in plant and its application in improving crop resistance and yield.Finally,the development and application prospects of CRISPR/Cas9 system in the innovation of herbicide-resistant-related germplasms were discussed.It is expected that the CRISPR/Cas9 system could provide a reference for the creating of new materials and the improvement of crop agronomic traits.关键词
CRISPR/Cas9原理/基因编辑/编辑效率/作物改良Key words
the principle of CRISPR/Cas9 system/gene editing/editing efficiency/crop improvement分类
农业科技引用本文复制引用
赵波,张余,张雨良..CRISPR/Cas9系统在植物育种中的应用[J].热带作物学报,2018,39(1):197-207,11.基金项目
公益性行业(农业)科研专项经费项目(No.201403075) （农业）
中国热带农业科学院院级“南繁育种与生物安全创新团队”项目(No.1630052017016). （No.1630052017016）
