山东医药2017，Vol.57Issue(47)：1-4,4.DOI:10.3969/j.issn.1002-266X.2017.47.001
葛黄颗粒含药血清对乙醇损伤的人肝细胞L-02保护作用观察及其机制探讨
Protective effect of Gehuang granule medicated serum on ethanol-induced injury of human hepatocytes L-02
摘要
Abstract
Objective To observe the effects of different dosages of Gehuang granule medicated serum (GHG) on the ethanol-induced injury of human hepatocytes L-02 and to explore its possible mechanism.Methods ① L-02 cells were divided into groups A,B,C,D,E,and F.Except the group F,the other groups were cultured in ethanol for 24 h,and the medium was taken away after the model was established.The groups D and F were added with the blank serum (10％ normal rat serum),the rest were added with the corresponding drug-containing serum:group A was added with 1.25％ GHG + 8.75％ normal rat serum,group B was added with 2.5％ GHG + 7.5％ normal rat serum,groups C was added with 5％ GHG +5％ normal rat serum,group E was added with 5％ metatoxin medicated serum +5％ normal rat serum and then we continued to culture the cells for 6,12,and 24 h.The activities of aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) in the supernatant of each group were compared.② The L-02 cells were inoculated into 2 × 104 cells/6-well plate,and then were divided into groups a,b,c,and d.In addition to the group d,the remaining groups were added with the ethanol modeling,after that,the medium was taken away.The groups b and d were added with the blank serum,group a was added with 5％ GHG,group c was added with 5％ metatoxin medicated serum and then we continued to culture the cells for 24 h.The levels of malondialdehyde (MDA),superoxide dismutase (SOD),glutathione peroxidase (GSH-px),reactive oxygen species (ROS),mitochondrial membrane potential (MMP),and adenosine triphosphate (ATP) were compared.Results Compared with group F,AST and LDH increased at each time point in the group D;compared with group D,AST and LDH in the group B decreased at 24 h,AST and LDH decreased at 12 and 24 h in the groups C and E,respectively;compared with group A,24-hour AST (24 h AST) decreased in the group B,24 h AST and 12 h LDH decreased in the group C,while AST and LDH decreased at 12 and 24 h in the group E;compared with the group B,AST and LDH decreased at 12 h in the group C and E;compared with the same group at 6 h,the 24 h AST in the group C decreased and the LDH decreased at 12 and 24 h;compared with the same group at 12 h,AST decreased at 24 h in the groups B,C,and E,and the LDH decreased at 24 h,all P ＜ 0.05.Compared with the group d,the levels of SOD,GSHpx,MMP,and ATP decreased,and MDA and ROS increased in the serum of group b (all P ＜ 0.05).Compared with the group b,the levels of SOD,GSH-px,MMP,and ATP increased,and MDA and ROS decreased in the groups a and c (all P ＜ 0.05).Conclusion GHG has a protective effect on ethanol-induced L-02 cell injury by regulating the level of intracellular oxidative stress and protecting mitochondrial function.关键词
葛黄颗粒/酒精性肝病/人肝细胞/L-02细胞/氧化损伤/线粒体功能Key words
Gehuang granules/ethanol/human hepatocytes/L-02 cells/oxidative damage/mitochondrial function分类
医药卫生引用本文复制引用
仁德芳,陈柚伶,郎涵,付裕,王洪连,魏嵋,王晓栋,李志..葛黄颗粒含药血清对乙醇损伤的人肝细胞L-02保护作用观察及其机制探讨[J].山东医药,2017,57(47):1-4,4.基金项目
四川省科技厅课题(2013SZ0148) （2013SZ0148）
泸州市科技局课题(2013LZLY-J24). （2013LZLY-J24）
